MicrobeMS IDENTIFICATION REPORT FROM MICROORGANISM MALDI-TOF MASS SPECTRA - OVERVIEW  

 

Database search parameters
database name:		230313_Full_DB_30Peaks_0.75.pkf
date / time of analysis:		15-Dec-2024 / 11:06:07
# of database entries:		1733
distance method:		Pearson scaling
use weightings:		distances obtained using peak intensities, weighting factor: 0.1
vary calibration parameters:		the complete set of calibration parameters was applied
# of variations:		343 ⇒ (3×2+1)³ variations of calibration parameters
rel width of m/z intervals (ppm):		800
calib variation range factor:		3
m/z range:		[1975 - 13162.5]
peak number ratio corr factor:		1
log score thresholds:		[2.5 - 2.75]
thresholds of rank list analyses:		[400 - 600]
mean score of rank list analyses:		769.9671

 
 
 
 

Short identification report for MALDI-ToF test spectra
Test spectra: ID's and quality test results	Summaries of score ranking lists	Score		Top entries of score ranking lists	Score
# 1 - 2013_06_13_Maren_Stämmler_0001	1 - Burkholderia pseudomallei	836.2		1 - Burkholderia pseudomallei A335-1	776.4
RKI MALDI sample 01, QUANDHIP EQAE ring t ...	2 - Burkholderia thailandensis	1.5		2 - Burkholderia pseudomallei PITT 521 041206RR1059	708.8
66.7 88.9 55.6 77.8 70.6	3 - Burkholderia mallei	0.2		3 - Burkholderia pseudomallei 03 04448 251109RR8920	626.1
# 2 - 2013_06_14_Maren_Stämmler_0001	1 - Burkholderia pseudomallei	851.9		1 - Burkholderia pseudomallei A335-1	804.9
RKI MALDI sample 01, QUANDHIP EQAE ring t ...	2 - Burkholderia mallei	0.7		2 - Burkholderia pseudomallei PITT 521 041206RR1059	736.7
66.7 100 44.4 77.8 69.4	3 - Burkholderia thailandensis	0		3 - Burkholderia pseudomallei Heckeshorn 041206RR1061	652.8
# 3 - 2013_06_13_Maren_Stämmler_0002	1 - Francisella tularensis ssp. holarctica	892.6		1 - Francisella tularensis ssp. holarctica 06T0001	841
RKI MALDI sample 02, QUANDHIP EQAE ring t ...	2 - Francisella tularensis ssp. tularensis	3.4		2 - Francisella tularensis ssp. holarctica A 1935-3	780.3
77.8 100 44.4 77.8 72.2	3 - Francisella tularensis ssp. mediasiatica	1.4		3 - Francisella tularensis ssp. holarctica Gaisky	760.8
# 4 - 2013_06_14_Maren_Stämmler_0002	1 - Francisella tularensis ssp. holarctica	906.5		1 - Francisella tularensis ssp. holarctica A 1935-3	835.3
RKI MALDI sample 02, QUANDHIP EQAE ring t ...	2 - Francisella tularensis ssp. tularensis	3.6		2 - Francisella tularensis ssp. holarctica Gaisky	812
55.6 77.8 33.3 66.7 56.1	3 - Francisella tularensis ssp. mediasiatica	1.7		3 - Francisella tularensis ssp. holarctica 06T0001	810.4
# 5 - 2013_06_13_Maren_Stämmler_0003	1 - Brucella canis	780		1 - Brucella canis A183-5	787.9
RKI MALDI sample 03, QUANDHIP EQAE ring t ...	2 - Brucella abortus	110.2		2 - Brucella canis A446-3	717.4
77.8 100 33.3 66.7 66.1	3 - Brucella melitensis	32		3 - Brucella canis 04 1 001 29	687.1
# 6 - 2013_06_14_Maren_Stämmler_0003	1 - Brucella canis	719.9		1 - Brucella canis A183-5	788.5
RKI MALDI sample 03, QUANDHIP EQAE ring t ...	2 - Brucella abortus	147.5		2 - Brucella canis A446-3	743.9
66.7 77.8 33.3 66.7 58.9	3 - Brucella melitensis	49.6		3 - Brucella abortus A104-10	681.7
# 7 - 2013_06_17_Maren_Stämmler_0001	1 - Bacillus anthracis	861.8		1 - Bacillus anthracis ATCC 4229 (RKI)	740.5
RKI MALDI sample 04, QUANDHIP EQAE ring t ...	2 - Bacillus cereus	0.5		2 - Bacillus anthracis unknown origin (A7, Beyer)	740.1
44.4 11.1 55.6 55.6 43.9	3 - n/a	0		3 - Bacillus anthracis unknown origin (A9, Beyer)	713.3
# 8 - 2013_06_14_Maren_Stämmler_0004	1 - Bacillus anthracis	832.8		1 - Bacillus anthracis unknown origin (A28, Beyer)	688.8
RKI MALDI sample 04, QUANDHIP EQAE ring t ...	2 - Bacillus cereus s.l.	1.1		2 - Bacillus anthracis unknown origin (A36, Beyer)	687.5
33.3 11.1 33.3 33.3 28.9	3 - Bacillus cereus	0		3 - Bacillus anthracis unknown origin (A73, Beyer)	682.8
# 9 - 2013_06_13_Maren_Stämmler_0004	1 - Bacillus anthracis	868.8		1 - Bacillus anthracis unknown origin (A61, Beyer)	764.8
RKI MALDI sample 04, QUANDHIP EQAE ring t ...	2 - Bacillus cereus	2.6		2 - Bacillus anthracis unknown origin (A27, Beyer)	739.3
44.4 11.1 55.6 55.6 43.9	3 - n/a	0		3 - Bacillus anthracis ATCC 4229 (RKI)	739.3
# 10 - 2013_06_13_Maren_Stämmler_0005	1 - Ochrobactrum anthropi	863.9		1 - Ochrobactrum anthropi DSM 20150, ATCC 11425	741.7
RKI MALDI sample 05, QUANDHIP EQAE ring t ...	2 - Brucella intermedia	150.9		2 - Ochrobactrum anthropi Ring trial A-269 08 A	715.2
66.7 77.8 55.6 66.7 65.6	3 - Burkholderia plantarii	74.8		3 - Brucella intermedia A1309	382.8
# 11 - 2013_06_14_Maren_Stämmler_0005	1 - Ochrobactrum anthropi	835.4		1 - Ochrobactrum anthropi DSM 20150, ATCC 11425	685
RKI MALDI sample 05, QUANDHIP EQAE ring t ...	2 - Brucella intermedia	141.9		2 - Ochrobactrum anthropi Ring trial A-269 08 A	683.8
55.6 33.3 55.6 66.7 53.9	3 - Brucella abortus	49.1		3 - Brucella intermedia A1309	353.4
# 12 - 2013_06_13_Maren_Stämmler_0006	1 - Yersinia pseudotuberculosis	794.6		1 - Yersinia pseudotuberculosis 29827	796.9
RKI MALDI sample 06, QUANDHIP EQAE ring t ...	2 - Yersinia pestis	66.5		2 - Yersinia pseudotuberculosis 29490	719.3
66.7 88.9 55.6 77.8 70.6	3 - Yersinia pestis	0.2		3 - Yersinia pseudotuberculosis 25743	693
# 13 - 2013_06_14_Maren_Stämmler_0006	1 - Yersinia pseudotuberculosis	865.6		1 - Yersinia pseudotuberculosis 29827	767.2
RKI MALDI sample 06, QUANDHIP EQAE ring t ...	2 - Yersinia pestis	10		2 - Yersinia pseudotuberculosis VI	766
55.6 55.6 55.6 77.8 61.1	3 - Yersinia pestis	0.2		3 - Yersinia pseudotuberculosis 29490	746.2
# 14 - 2013_06_13_Maren_Stämmler_0007	1 - Burkholderia mallei	823.1		1 - Burkholderia mallei Mukteswar 290103RR0041	699.8
RKI MALDI sample 07, QUANDHIP EQAE ring t ...	2 - Burkholderia pseudomallei	0		2 - Burkholderia mallei 3708	659.7
66.7 100 44.4 77.8 69.4	3 - n/a	0		3 - Burkholderia mallei Dubai 7	650.3
# 15 - 2013_06_14_Maren_Stämmler_0007	1 - Burkholderia mallei	781.5		1 - Burkholderia mallei Mukteswar 290103RR0041	714.3
RKI MALDI sample 07, QUANDHIP EQAE ring t ...	2 - Burkholderia pseudomallei	49		2 - Burkholderia mallei Dubai 7	678.1
66.7 100 44.4 77.8 69.4	3 - n/a	0		3 - Burkholderia mallei 3708	657.8
# 16 - 2013_06_17_Maren_Stämmler_0002	1 - Burkholderia thailandensis	816.6		1 - Burkholderia thailandensis E163	669.8
RKI MALDI sample 08, QUANDHIP EQAE ring t ...	2 - Burkholderia pseudomallei	0		2 - Burkholderia thailandensis E125	668.1
44.4 44.4 44.4 55.6 47.2	3 - n/a	0		3 - Burkholderia thailandensis E207	645.6
# 17 - 2013_06_13_Maren_Stämmler_0008	1 - Burkholderia thailandensis	880.3		1 - Burkholderia thailandensis DSM 13276	768.2
RKI MALDI sample 08, QUANDHIP EQAE ring t ...	2 - Burkholderia mallei	0		2 - Burkholderia thailandensis E202	765.6
55.6 77.8 55.6 66.7 62.8	3 - n/a	0		3 - Burkholderia thailandensis E184	764.2
# 18 - 2013_06_14_Maren_Stämmler_0008	1 - Burkholderia thailandensis	872.6		1 - Burkholderia thailandensis DSM 13276	767.2
RKI MALDI sample 08, QUANDHIP EQAE ring t ...	2 - Burkholderia pseudomallei	0		2 - Burkholderia thailandensis E125	737.2
44.4 33.3 55.6 66.7 51.1	3 - Burkholderia mallei	0		3 - Burkholderia thailandensis E067	736.2
# 19 - 2013_06_17_Maren_Stämmler_0003	1 - Yersinia pestis	385.7		1 - Yersinia pestis O3-01500	350.6
RKI MALDI sample 09, QUANDHIP EQAE ring t ...	2 - Yersinia similis	55.9		2 - Yersinia pestis O3-01501	302.3
77.8 33.3 22.2 33.3 41.1	3 - Bacillus anthracis	43		3 - Yersinia pestis CCUG EV 76, CCUG 32133	288.5
# 20 - 2013_06_13_Maren_Stämmler_0009	1 - Yersinia pestis	400		1 - Yersinia pestis O3-01501	368.8
RKI MALDI sample 09, QUANDHIP EQAE ring t ...	2 - Bacillus anthracis	80.8		2 - Yersinia pestis O3-01500	334.9
77.8 77.8 22.2 55.6 55.6	3 - Yersinia similis	48.6		3 - Bacillus anthracis unknown origin (A122, Beyer)	329
# 21 - 2013_06_14_Maren_Stämmler_0009	1 - Yersinia pestis	385		1 - Yersinia pestis O3-01501	364.6
RKI MALDI sample 09, QUANDHIP EQAE ring t ...	2 - Bacillus anthracis	80.9		2 - Yersinia pestis O3-01500	329.7
77.8 55.6 11.1 55.6 47.8	3 - Yersinia similis	78.1		3 - Bacillus anthracis unknown origin (A122, Beyer)	329.4
# 22 - 2013_06_17_Maren_Stämmler_0004	1 - Bacillus thuringiensis	707.9		1 - Bacillus thuringiensis DSM 2046 (B188, Beyer)	552.4
RKI MALDI sample 10, QUANDHIP EQAE ring t ...	2 - Bacillus cereus	11.5		2 - Bacillus thuringiensis DSM 2046	526.6
33.3 11.1 44.4 44.4 35	3 - Bacillus anthracis	1.5		3 - Bacillus thuringiensis DSM 6890	515.6
# 23 - 2013_06_13_Maren_Stämmler_0010	1 - Bacillus thuringiensis	804.4		1 - Bacillus thuringiensis DSM 6890	703.6
RKI MALDI sample 10, QUANDHIP EQAE ring t ...	2 - Bacillus toyonensis	8.2		2 - Bacillus thuringiensis DSM 350	644.9
33.3 11.1 44.4 55.6 37.8	3 - Bacillus anthracis	2.3		3 - Bacillus thuringiensis DSM 2046 (B188, Beyer)	641.6
# 24 - 2013_06_14_Maren_Stämmler_0010	1 - Bacillus thuringiensis	712.1		1 - Bacillus thuringiensis DSM 350	581.9
RKI MALDI sample 10, QUANDHIP EQAE ring t ...	2 - Bacillus anthracis	13.9		2 - Bacillus thuringiensis DSM 6890	547.8
33.3 11.1 33.3 33.3 28.9	3 - Bacillus toyonensis	3.8		3 - Bacillus thuringiensis WS 2621	510.8

 
 
 
 

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# 1 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0001'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0001
type:		Measurement 01
NCBI ID (primary):		28450
NCBI ID (secondary):		28450
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia pseudomallei A101-10: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T13:52:45.062+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_01/0_H3/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia pseudomallei	836.1696	2.9223	28450	20
2	Burkholderia thailandensis	1.5338	0.18578	57975	15
3	Burkholderia mallei	0.19275	-0.71501	13373	33

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia pseudomallei A335-1	776.4395	2.8901	dbspec-13-Mar-2023-12-34-35.273	RKI ZBS2
2	Burkholderia pseudomallei PITT 521 041206RR1059	708.8101	2.8505	dbspec-13-Mar-2023-12-36-9.146	Dr. Tomaso (FLI Jena)
3	Burkholderia pseudomallei 03 04448 251109RR8920	626.1187	2.7967	dbspec-13-Mar-2023-12-33-32.921	Dr. Tomaso (FLI Jena)
4	Burkholderia pseudomallei Heckeshorn 041206RR1061	618.7753	2.7915	dbspec-13-Mar-2023-12-35-10.944	Dr. Tomaso (FLI Jena)
5	Burkholderia pseudomallei 3463	597.0976	2.776	dbspec-13-Mar-2023-12-35-3.898	RKI ZBS6
6	Burkholderia pseudomallei 10274	573.6714	2.7587	dbspec-13-Mar-2023-12-34-58.71	RKI ZBS6
7	Burkholderia pseudomallei 03 04450 060406RR0740	557.0811	2.7459	dbspec-13-Mar-2023-12-33-58.03	Dr. Tomaso (FLI Jena)
8	Burkholderia pseudomallei CCUG 15649	544.2299	2.7358	dbspec-13-Mar-2023-12-34-50.132	RKI ZBS6
9	Burkholderia pseudomallei CCUG 15651	542.2729	2.7342	dbspec-13-Mar-2023-12-34-54.445	RKI ZBS6
10	Burkholderia pseudomallei CCUG 15648	542.2587	2.7342	dbspec-13-Mar-2023-12-34-46.289	RKI ZBS6
11	Burkholderia thailandensis E153	534.328	2.7278	dbspec-13-Mar-2023-12-43-24.09	RKI ZBS6
12	Burkholderia thailandensis E143	530.9122	2.725	dbspec-13-Mar-2023-12-43-19.996	RKI ZBS6
13	Burkholderia mallei NCTC 10247 041206RR1056	526.7727	2.7216	dbspec-13-Mar-2023-12-28-54.802	Dr. Tomaso (FLI Jena)
14	Burkholderia thailandensis E184	525.8289	2.7208	dbspec-13-Mar-2023-12-43-32.106	RKI ZBS6
15	Burkholderia mallei BfR M1 290103RR0043	524.6017	2.7198	dbspec-13-Mar-2023-12-26-31.59	Dr. Tomaso (FLI Jena)
16	Burkholderia mallei ATCC 23344 300102RR0118	523.3953	2.7188	dbspec-13-Mar-2023-12-22-41.157	Dr. Tomaso (FLI Jena)
17	Burkholderia thailandensis E131	521.9192	2.7176	dbspec-13-Mar-2023-12-43-15.778	RKI ZBS6
18	Burkholderia thailandensis E049	521.6082	2.7173	dbspec-13-Mar-2023-12-42-52.075	RKI ZBS6
19	Burkholderia pseudomallei ring trial A-269 01 A	519.8388	2.7159	dbspec-13-Mar-2023-12-33-29.89	RKI ZBS2|ZBS6
20	Burkholderia mallei BfR 242 300102RR0106	504.0388	2.7025	dbspec-13-Mar-2023-12-24-37.295	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 2 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0001'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0001
type:		Measurement 02
NCBI ID (primary):		28450
NCBI ID (secondary):		28450
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia pseudomallei A101-10: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:27:14.015+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_01/0_H4/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia pseudomallei	851.8834	2.9304	28450	20
2	Burkholderia mallei	0.71759	-0.14412	13373	33
3	Burkholderia thailandensis	2.6874e-06	-5.5707	57975	15

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia pseudomallei A335-1	804.9022	2.9057	dbspec-13-Mar-2023-12-34-35.273	RKI ZBS2
2	Burkholderia pseudomallei PITT 521 041206RR1059	736.7184	2.8673	dbspec-13-Mar-2023-12-36-9.146	Dr. Tomaso (FLI Jena)
3	Burkholderia pseudomallei Heckeshorn 041206RR1061	652.7795	2.8148	dbspec-13-Mar-2023-12-35-10.944	Dr. Tomaso (FLI Jena)
4	Burkholderia pseudomallei 03 04448 251109RR8920	624.8182	2.7958	dbspec-13-Mar-2023-12-33-32.921	Dr. Tomaso (FLI Jena)
5	Burkholderia pseudomallei CCUG 15648	605.2762	2.782	dbspec-13-Mar-2023-12-34-46.289	RKI ZBS6
6	Burkholderia pseudomallei ring trial A-269 01 A	594.8047	2.7744	dbspec-13-Mar-2023-12-33-29.89	RKI ZBS2|ZBS6
7	Burkholderia pseudomallei 03 04450 060406RR0740	576.7976	2.761	dbspec-13-Mar-2023-12-33-58.03	Dr. Tomaso (FLI Jena)
8	Burkholderia pseudomallei CCUG 15649	572.1573	2.7575	dbspec-13-Mar-2023-12-34-50.132	RKI ZBS6
9	Burkholderia pseudomallei CCUG 15651	570.2446	2.7561	dbspec-13-Mar-2023-12-34-54.445	RKI ZBS6
10	Burkholderia pseudomallei 10274	566.0301	2.7528	dbspec-13-Mar-2023-12-34-58.71	RKI ZBS6
11	Burkholderia pseudomallei 3463	565.5537	2.7525	dbspec-13-Mar-2023-12-35-3.898	RKI ZBS6
12	Burkholderia mallei BfR 242 300102RR0106	560.6815	2.7487	dbspec-13-Mar-2023-12-24-37.295	Dr. Tomaso (FLI Jena)
13	Burkholderia mallei BfR 242 041206RR1050	560.1606	2.7483	dbspec-13-Mar-2023-12-24-4.577	Dr. Tomaso (FLI Jena)
14	Burkholderia mallei NCTC 10247 041206RR1056	555.6501	2.7448	dbspec-13-Mar-2023-12-28-54.802	Dr. Tomaso (FLI Jena)
15	Burkholderia mallei Dubai 7	552.5252	2.7424	dbspec-13-Mar-2023-12-27-36.994	Dr. Tomaso (FLI Jena)
16	Burkholderia mallei BfR M1 290103RR0043	552.274	2.7422	dbspec-13-Mar-2023-12-26-31.59	Dr. Tomaso (FLI Jena)
17	Burkholderia pseudomallei NCTC 1688 041206RR1062	552.1597	2.7421	dbspec-13-Mar-2023-12-35-39.413	Dr. Tomaso (FLI Jena)
18	Burkholderia mallei ATCC 23344 300102RR0118	551.2001	2.7413	dbspec-13-Mar-2023-12-22-41.157	Dr. Tomaso (FLI Jena)
19	Burkholderia thailandensis E184	550.187	2.7405	dbspec-13-Mar-2023-12-43-32.106	RKI ZBS6
20	Burkholderia pseudomallei PITT 5691 041206RR1064	547.4006	2.7383	dbspec-13-Mar-2023-12-36-28.661	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 3 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0002'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0002
type:		Measurement 01
NCBI ID (primary):		119857
NCBI ID (secondary):		119857
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Heart cysteine agar (HCA)
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Francisella tularensis ssp. holarctica Ft 32: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:04:41.578+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_02/0_H5/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Francisella tularensis ssp. holarctica	892.6283	2.9507	119857	9
2	Francisella tularensis ssp. tularensis	3.3574	0.52601	119856	1
3	Francisella tularensis ssp. mediasiatica	1.4197	0.1522	135248	3
4	Francisella tularensis ssp. novicida	0.42396	-0.37267	264	4
5	Morganella morganii ssp. sibonii	0.0043158	-2.3649	180435	2

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Francisella tularensis ssp. holarctica 06T0001	840.9696	2.9248	dbspec-13-Mar-2023-13-9-54.532	Dr. Tomaso (FLI Jena)
2	Francisella tularensis ssp. holarctica A 1935-3	780.3155	2.8923	dbspec-13-Mar-2023-13-10-51.952	RKI ZBS2|ZBS6
3	Francisella tularensis ssp. holarctica Gaisky	760.7607	2.8812	dbspec-13-Mar-2023-13-11-29.202	Dr. Tomaso (FLI Jena)
4	Francisella tularensis ssp. holarctica A 1935-1	752.587	2.8766	dbspec-13-Mar-2023-13-10-37.75	RKI ZBS2|ZBS6
5	Francisella tularensis ssp. holarctica W 1468	690.7737	2.8393	dbspec-13-Mar-2023-13-13-2.34	Dr. Tomaso (FLI Jena)
6	Francisella tularensis ssp. holarctica W 2903	681.3396	2.8334	dbspec-13-Mar-2023-13-13-29.777	Dr. Tomaso (FLI Jena)
7	Francisella tularensis ssp. holarctica Ft32, FSC 200	679.3812	2.8321	dbspec-13-Mar-2023-13-11-14.499	RKI ZBS2|ZBS6
8	Francisella tularensis ssp. holarctica LVS	675.0824	2.8294	dbspec-13-Mar-2023-13-12-7.341	Dr. Tomaso (FLI Jena)
9	Francisella tularensis ssp. holarctica A466-1	573.0931	2.7582	dbspec-13-Mar-2023-13-11-6.921	RKI ZBS2|ZBS6
10	Francisella tularensis ssp. tularensis FSC 237	464.4837	2.667	dbspec-13-Mar-2023-13-17-1.007	Dr. Tomaso (FLI Jena)
11	Francisella tularensis ssp. mediasiatica Ft31, FSC 148	432.7493	2.6362	dbspec-13-Mar-2023-13-15-14.415	RKI ZBS2|ZBS6
12	Francisella tularensis ssp. novicida F059	376.0886	2.5753	dbspec-13-Mar-2023-13-16-4.664	Dr. Tomaso (FLI Jena)
13	Francisella tularensis ssp. mediasiatica F063	358.0766	2.554	dbspec-13-Mar-2023-13-14-0.979	Dr. Tomaso (FLI Jena)
14	Francisella tularensis ssp. mediasiatica F064	356.7433	2.5524	dbspec-13-Mar-2023-13-14-36.885	Dr. Tomaso (FLI Jena)
15	Francisella tularensis ssp. novicida Ft26	338.0801	2.529	dbspec-13-Mar-2023-13-16-52.913	Dr. Tomaso (FLI Jena)
16	Morganella morganii ssp. sibonii DSM 6675	295.4453	2.4705	dbspec-13-Mar-2023-13-24-44.075	RKI ZBS6
17	Francisella tularensis ssp. novicida F048	290.9115	2.4638	dbspec-13-Mar-2023-13-15-25.009	Dr. Tomaso (FLI Jena)
18	Morganella morganii ssp. morganii DSM 30164	289.228	2.4612	dbspec-13-Mar-2023-13-24-32.809	RKI ZBS6
19	Francisella tularensis ssp. novicida Ring trial A-269 05 A	278.7077	2.4451	dbspec-13-Mar-2023-13-15-21.868	RKI ZBS2|ZBS6
20	Vibrio harveyi A2017	263.8506	2.4214	dbspec-13-Mar-2023-13-57-43.844	RKI ZBS6

 
 
 
 

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# 4 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0002'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0002
type:		Measurement 02
NCBI ID (primary):		119857
NCBI ID (secondary):		119857
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Heart cysteine agar (HCA)
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Francisella tularensis ssp. holarctica Ft 32: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:33:44.531+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_02/0_H6/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Francisella tularensis ssp. holarctica	906.4706	2.9574	119857	9
2	Francisella tularensis ssp. tularensis	3.5888	0.55495	119856	1
3	Francisella tularensis ssp. mediasiatica	1.7255	0.23691	135248	3
4	Francisella tularensis ssp. novicida	0.15558	-0.80805	264	4
5	Morganella morganii ssp. sibonii	0.0042203	-2.3747	180435	2

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Francisella tularensis ssp. holarctica A 1935-3	835.3327	2.9219	dbspec-13-Mar-2023-13-10-51.952	RKI ZBS2|ZBS6
2	Francisella tularensis ssp. holarctica Gaisky	812.0346	2.9096	dbspec-13-Mar-2023-13-11-29.202	Dr. Tomaso (FLI Jena)
3	Francisella tularensis ssp. holarctica 06T0001	810.375	2.9087	dbspec-13-Mar-2023-13-9-54.532	Dr. Tomaso (FLI Jena)
4	Francisella tularensis ssp. holarctica A 1935-1	779.6302	2.8919	dbspec-13-Mar-2023-13-10-37.75	RKI ZBS2|ZBS6
5	Francisella tularensis ssp. holarctica Ft32, FSC 200	758.7376	2.8801	dbspec-13-Mar-2023-13-11-14.499	RKI ZBS2|ZBS6
6	Francisella tularensis ssp. holarctica W 1468	744.7473	2.872	dbspec-13-Mar-2023-13-13-2.34	Dr. Tomaso (FLI Jena)
7	Francisella tularensis ssp. holarctica LVS	725.9574	2.8609	dbspec-13-Mar-2023-13-12-7.341	Dr. Tomaso (FLI Jena)
8	Francisella tularensis ssp. holarctica W 2903	704.5741	2.8479	dbspec-13-Mar-2023-13-13-29.777	Dr. Tomaso (FLI Jena)
9	Francisella tularensis ssp. holarctica A466-1	607.8238	2.7838	dbspec-13-Mar-2023-13-11-6.921	RKI ZBS2|ZBS6
10	Francisella tularensis ssp. tularensis FSC 237	516.3485	2.7129	dbspec-13-Mar-2023-13-17-1.007	Dr. Tomaso (FLI Jena)
11	Francisella tularensis ssp. mediasiatica Ft31, FSC 148	460.6656	2.6634	dbspec-13-Mar-2023-13-15-14.415	RKI ZBS2|ZBS6
12	Francisella tularensis ssp. mediasiatica F064	346.2853	2.5394	dbspec-13-Mar-2023-13-14-36.885	Dr. Tomaso (FLI Jena)
13	Francisella tularensis ssp. novicida F059	326.326	2.5137	dbspec-13-Mar-2023-13-16-4.664	Dr. Tomaso (FLI Jena)
14	Francisella tularensis ssp. mediasiatica F063	322.7376	2.5088	dbspec-13-Mar-2023-13-14-0.979	Dr. Tomaso (FLI Jena)
15	Francisella tularensis ssp. novicida Ft26	301.1785	2.4788	dbspec-13-Mar-2023-13-16-52.913	Dr. Tomaso (FLI Jena)
16	Morganella morganii ssp. sibonii DSM 6675	288.4519	2.4601	dbspec-13-Mar-2023-13-24-44.075	RKI ZBS6
17	Morganella morganii ssp. morganii DSM 30164	282.4526	2.4509	dbspec-13-Mar-2023-13-24-32.809	RKI ZBS6
18	Francisella tularensis ssp. novicida Ring trial A-269 05 A	280.6476	2.4482	dbspec-13-Mar-2023-13-15-21.868	RKI ZBS2|ZBS6
19	Francisella tularensis ssp. novicida F048	275.3769	2.4399	dbspec-13-Mar-2023-13-15-25.009	Dr. Tomaso (FLI Jena)
20	Klebsiella oxytoca CB4063	265.4786	2.424	dbspec-13-Mar-2023-13-19-33.035	RKI ZBS2|ZBS6

 
 
 
 

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# 5 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0003'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0003
type:		Measurement 01
NCBI ID (primary):		36855
NCBI ID (secondary):		36855
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Brucella canis A138-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:11:32.906+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_03/0_H7/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Brucella canis	779.9962	2.8921	36855	5
2	Brucella abortus	110.182	2.0421	235	16
3	Brucella melitensis	31.9573	1.5046	29459	9
4	Brucella neotomae	0.082028	-1.086	29460	3
5	Brucella ovis	0.0048968	-2.3101	236	4

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Brucella canis A183-5	787.8713	2.8965	dbspec-13-Mar-2023-11-50-6.184	RKI ZBS2|ZBS6
2	Brucella canis A446-3	717.3606	2.8557	dbspec-13-Mar-2023-11-50-13.199	RKI ZBS2
3	Brucella canis 04 1 001 29	687.1424	2.837	dbspec-13-Mar-2023-11-49-34.403	Dr. Tomaso (FLI Jena)
4	Brucella abortus A104-10	682.9313	2.8344	dbspec-13-Mar-2023-11-46-47.141	RKI ZBS2
5	Brucella canis R 6 66 03RB0214 3	672.009	2.8274	dbspec-13-Mar-2023-11-50-20.215	Dr. Tomaso (FLI Jena)
6	Brucella melitensis A146-13	643.6812	2.8087	dbspec-13-Mar-2023-11-55-5.912	RKI ZBS2
7	Brucella abortus 2 Strain 86 8 59	627.5014	2.7976	dbspec-13-Mar-2023-11-44-22.238	Dr. Tomaso (FLI Jena)
8	Brucella abortus 5 Strain B3196 07RB1201	625.0283	2.7959	dbspec-13-Mar-2023-11-45-16.503	Dr. Tomaso (FLI Jena)
9	Brucella canis (BM385)	621.5102	2.7934	dbspec-13-Mar-2023-11-49-5.154	TNO Netherlands
10	Brucella melitensis 02 2 021 14	617.8948	2.7909	dbspec-13-Mar-2023-11-54-0.366	Dr. Tomaso (FLI Jena)
11	Brucella abortus 9 Strain C68 07RB1203	599.053	2.7775	dbspec-13-Mar-2023-11-46-15.283	Dr. Tomaso (FLI Jena)
12	Brucella abortus 01 1 001 1	599.0212	2.7774	dbspec-13-Mar-2023-11-43-0.256	Dr. Tomaso (FLI Jena)
13	Brucella abortus Ring trial A-269 06 A	595.9551	2.7752	dbspec-13-Mar-2023-11-46-44.438	RKI ZBS2|ZBS6
14	Brucella neotomae A148-7	579.0704	2.7627	dbspec-13-Mar-2023-11-58-50.844	RKI ZBS2|ZBS6
15	Brucella abortus S19	575.6401	2.7602	dbspec-13-Mar-2023-11-47-19.344	RKI FG14
16	Brucella ovis 63 290 03RB0213 3	574.668	2.7594	dbspec-13-Mar-2023-12-0-31.733	Dr. Tomaso (FLI Jena)
17	Brucella abortus 6 Strain 870 07RB1202	568.42	2.7547	dbspec-13-Mar-2023-11-45-47.658	Dr. Tomaso (FLI Jena)
18	Brucella melitensis 02 1 014 10	564.5446	2.7517	dbspec-13-Mar-2023-11-53-30.117	Dr. Tomaso (FLI Jena)
19	Brucella sp. A 1755_25	564.1664	2.7514	dbspec-13-Mar-2023-12-2-29.121	RKI ZBS6
20	Brucella melitensis 02 3 022 17	563.3816	2.7508	dbspec-13-Mar-2023-11-54-33.584	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 6 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0003'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0003
type:		Measurement 02
NCBI ID (primary):		36855
NCBI ID (secondary):		36855
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Brucella canis A138-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:42:40.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_03/0_H8/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Brucella canis	719.889	2.8573	36855	5
2	Brucella abortus	147.5041	2.1688	235	16
3	Brucella melitensis	49.5507	1.695	29459	9
4	Brucella ovis	0.54447	-0.26403	236	4
5	Brucella neotomae	0.2386	-0.62232	29460	3

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Brucella canis A183-5	788.5113	2.8968	dbspec-13-Mar-2023-11-50-6.184	RKI ZBS2|ZBS6
2	Brucella canis A446-3	743.898	2.8715	dbspec-13-Mar-2023-11-50-13.199	RKI ZBS2
3	Brucella abortus A104-10	681.7162	2.8336	dbspec-13-Mar-2023-11-46-47.141	RKI ZBS2
4	Brucella canis 04 1 001 29	656.5417	2.8173	dbspec-13-Mar-2023-11-49-34.403	Dr. Tomaso (FLI Jena)
5	Brucella melitensis A146-13	644.7847	2.8094	dbspec-13-Mar-2023-11-55-5.912	RKI ZBS2
6	Brucella canis R 6 66 03RB0214 3	643.1759	2.8083	dbspec-13-Mar-2023-11-50-20.215	Dr. Tomaso (FLI Jena)
7	Brucella canis (BM385)	620.4677	2.7927	dbspec-13-Mar-2023-11-49-5.154	TNO Netherlands
8	Brucella abortus 9 Strain C68 07RB1203	600.4421	2.7785	dbspec-13-Mar-2023-11-46-15.283	Dr. Tomaso (FLI Jena)
9	Brucella abortus 5 Strain B3196 07RB1201	598.7329	2.7772	dbspec-13-Mar-2023-11-45-16.503	Dr. Tomaso (FLI Jena)
10	Brucella abortus 2 Strain 86 8 59	596.6854	2.7757	dbspec-13-Mar-2023-11-44-22.238	Dr. Tomaso (FLI Jena)
11	Brucella abortus Ring trial A-269 06 A	596.5915	2.7757	dbspec-13-Mar-2023-11-46-44.438	RKI ZBS2|ZBS6
12	Brucella ovis 63 290 03RB0213 3	583.7466	2.7662	dbspec-13-Mar-2023-12-0-31.733	Dr. Tomaso (FLI Jena)
13	Brucella neotomae A148-7	578.5517	2.7623	dbspec-13-Mar-2023-11-58-50.844	RKI ZBS2|ZBS6
14	Brucella abortus 6 Strain 870 07RB1202	571.6227	2.7571	dbspec-13-Mar-2023-11-45-47.658	Dr. Tomaso (FLI Jena)
15	Brucella abortus 01 1 001 1	566.8622	2.7535	dbspec-13-Mar-2023-11-43-0.256	Dr. Tomaso (FLI Jena)
16	Brucella sp. A 1755_25	563.2655	2.7507	dbspec-13-Mar-2023-12-2-29.121	RKI ZBS6
17	Brucella abortus 1 544 03RB0215 3	562.2413	2.7499	dbspec-13-Mar-2023-11-43-30.255	Dr. Tomaso (FLI Jena)
18	Brucella abortus biovar 3 (BM369)	559.1115	2.7475	dbspec-13-Mar-2023-11-48-3.374	TNO Netherlands
19	Brucella abortus biovar 3 (BM382)	558.7199	2.7472	dbspec-13-Mar-2023-11-48-34.42	TNO Netherlands
20	Brucella ovis A183-7	551.7987	2.7418	dbspec-13-Mar-2023-12-0-52.686	RKI ZBS2

 
 
 
 

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# 7 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0001'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0001
type:		Measurement 01
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:21:01.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_E19/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus anthracis	861.8239	2.9354	1392	130
2	Bacillus cereus	0.53246	-0.27371	1392.1	6
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus anthracis ATCC 4229 (RKI)	740.5404	2.8695	dbspec-13-Mar-2023-10-24-3.44	RKI ZBS2
2	Bacillus anthracis unknown origin (A7, Beyer)	740.1116	2.8693	dbspec-13-Mar-2023-10-32-18.4	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
3	Bacillus anthracis unknown origin (A9, Beyer)	713.2792	2.8533	dbspec-13-Mar-2023-10-33-14.227	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
4	Bacillus anthracis unknown origin (A18, Beyer)	712.1002	2.8525	dbspec-13-Mar-2023-10-29-14.326	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
5	Bacillus anthracis unknown origin (A27, Beyer)	712.0405	2.8525	dbspec-13-Mar-2023-10-29-46.404	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
6	Bacillus anthracis Sterne vaccine (A118, Beyer)	708.2244	2.8502	dbspec-13-Mar-2023-10-24-54.204	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
7	Bacillus anthracis unknown origin (A61, Beyer)	707.4565	2.8497	dbspec-13-Mar-2023-10-31-44.87	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
8	Bacillus anthracis unknown origin (A10, Beyer)	686.4696	2.8366	dbspec-13-Mar-2023-10-26-50.642	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
9	Bacillus anthracis unknown origin (A28, Beyer)	686.3614	2.8366	dbspec-13-Mar-2023-10-29-51.2	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
10	Bacillus anthracis unknown origin (A73, Beyer)	683.6095	2.8348	dbspec-13-Mar-2023-10-32-23.963	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
11	Bacillus anthracis unknown origin (A121, Beyer)	683.2221	2.8346	dbspec-13-Mar-2023-10-28-29.046	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
12	Bacillus cereus DSM 8438 (B248, Beyer)	682.588	2.8342	dbspec-13-Mar-2023-10-41-8.42	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis unknown origin, black (A89, Beyer)	680.9268	2.8331	dbspec-13-Mar-2023-10-33-30.649	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus anthracis ATCC 14578 (A70, Beyer)	679.5748	2.8322	dbspec-13-Mar-2023-10-23-52.222	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
15	Bacillus anthracis unknown origin (A62, Beyer)	678.3569	2.8315	dbspec-13-Mar-2023-10-31-50.057	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
16	Bacillus anthracis unknown origin (A32, Beyer)	676.6017	2.8303	dbspec-13-Mar-2023-10-30-8.372	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus anthracis ATCC 14578 (A71, Beyer)	676.5864	2.8303	dbspec-13-Mar-2023-10-23-57.94	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Bacillus anthracis Sterne vaccine (A1, Beyer)	675.2538	2.8295	dbspec-13-Mar-2023-10-24-48.751	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
19	Bacillus anthracis unknown origin (A35, Beyer)	654.2616	2.8158	dbspec-13-Mar-2023-10-30-25.434	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
20	Bacillus anthracis unknown origin (A63, Beyer)	653.8726	2.8155	dbspec-13-Mar-2023-10-31-56.088	RKI ZBS6, Dr. Beyer (Uni Hohenheim)

 
 
 
 

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# 8 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0004'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0004
type:		Measurement 02
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:52:26.953+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_H10/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus anthracis	832.7555	2.9205	1392	130
2	Bacillus cereus s.l.	1.1492	0.060379	86661	22
3	Bacillus cereus	3.0161e-06	-5.5206	1392.1	6

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus anthracis unknown origin (A28, Beyer)	688.8037	2.8381	dbspec-13-Mar-2023-10-29-51.2	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
2	Bacillus anthracis unknown origin (A36, Beyer)	687.4709	2.8373	dbspec-13-Mar-2023-10-30-31.199	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
3	Bacillus anthracis unknown origin (A73, Beyer)	682.8084	2.8343	dbspec-13-Mar-2023-10-32-23.963	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
4	Bacillus anthracis unknown origin (A18, Beyer)	679.5772	2.8322	dbspec-13-Mar-2023-10-29-14.326	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
5	Bacillus anthracis unknown origin (A10, Beyer)	661.0087	2.8202	dbspec-13-Mar-2023-10-26-50.642	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
6	Bacillus anthracis unknown origin (A16, Beyer)	658.4765	2.8185	dbspec-13-Mar-2023-10-29-8.514	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
7	Bacillus anthracis ATCC 14185 (A2, Beyer)	655.389	2.8165	dbspec-13-Mar-2023-10-23-50.753	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
8	Bacillus anthracis unknown origin (A9, Beyer)	653.1382	2.815	dbspec-13-Mar-2023-10-33-14.227	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
9	Bacillus anthracis unknown origin (A32, Beyer)	651.8541	2.8142	dbspec-13-Mar-2023-10-30-8.372	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
10	Bacillus anthracis unknown origin (A7, Beyer)	650.6419	2.8133	dbspec-13-Mar-2023-10-32-18.4	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
11	Bacillus cereus s.l. unknown origin (B340, Beyer)	646.5814	2.8106	dbspec-13-Mar-2023-10-48-12.577	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
12	Bacillus anthracis unknown origin (A5, Beyer)	631.8379	2.8006	dbspec-13-Mar-2023-10-31-22.823	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis ATCC 14578 (A70, Beyer)	626.2732	2.7968	dbspec-13-Mar-2023-10-23-52.222	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus anthracis unknown origin (A3, Beyer)	626.0613	2.7966	dbspec-13-Mar-2023-10-30-2.45	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
15	Bacillus anthracis unknown origin (A63, Beyer)	624.9427	2.7958	dbspec-13-Mar-2023-10-31-56.088	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
16	Bacillus anthracis unknown origin (A113, Beyer)	624.3461	2.7954	dbspec-13-Mar-2023-10-28-10.734	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus anthracis Sterne vaccine (A1, Beyer)	623.689	2.795	dbspec-13-Mar-2023-10-24-48.751	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Bacillus anthracis unknown origin (A27, Beyer)	623.3698	2.7947	dbspec-13-Mar-2023-10-29-46.404	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
19	Bacillus cereus DSM 8438 (B248, Beyer)	623.0506	2.7945	dbspec-13-Mar-2023-10-41-8.42	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
20	Bacillus anthracis unknown origin, black (A89, Beyer)	621.8479	2.7937	dbspec-13-Mar-2023-10-33-30.649	RKI ZBS6, Dr. Beyer (Uni Hohenheim)

 
 
 
 

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# 9 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0004'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0004
type:		Measurement 03
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:19:29.671+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_H9/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus anthracis	868.7979	2.9389	1392	130
2	Bacillus cereus	2.6483	0.42297	1392.1	6
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus anthracis unknown origin (A61, Beyer)	764.7919	2.8835	dbspec-13-Mar-2023-10-31-44.87	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
2	Bacillus anthracis unknown origin (A27, Beyer)	739.338	2.8688	dbspec-13-Mar-2023-10-29-46.404	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
3	Bacillus anthracis ATCC 4229 (RKI)	739.3352	2.8688	dbspec-13-Mar-2023-10-24-3.44	RKI ZBS2
4	Bacillus anthracis unknown origin (A7, Beyer)	738.867	2.8686	dbspec-13-Mar-2023-10-32-18.4	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
5	Bacillus anthracis unknown origin (A9, Beyer)	710.8349	2.8518	dbspec-13-Mar-2023-10-33-14.227	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
6	Bacillus anthracis unknown origin (A73, Beyer)	709.1301	2.8507	dbspec-13-Mar-2023-10-32-23.963	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
7	Bacillus anthracis Sterne vaccine (A1, Beyer)	703.7227	2.8474	dbspec-13-Mar-2023-10-24-48.751	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
8	Bacillus anthracis animal (A131, Beyer)	700.1587	2.8452	dbspec-13-Mar-2023-10-25-34.237	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
9	Bacillus anthracis unknown origin (A28, Beyer)	684.4259	2.8353	dbspec-13-Mar-2023-10-29-51.2	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
10	Bacillus cereus DSM 8438 (B248, Beyer)	681.0153	2.8332	dbspec-13-Mar-2023-10-41-8.42	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
11	Bacillus anthracis unknown origin (A18, Beyer)	679.5295	2.8322	dbspec-13-Mar-2023-10-29-14.326	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
12	Bacillus anthracis unknown origin (A3, Beyer)	678.3031	2.8314	dbspec-13-Mar-2023-10-30-2.45	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis unknown origin, black (A89, Beyer)	677.9352	2.8312	dbspec-13-Mar-2023-10-33-30.649	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus anthracis ATCC 14578 (A70, Beyer)	677.3211	2.8308	dbspec-13-Mar-2023-10-23-52.222	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
15	Bacillus anthracis unknown origin (A63, Beyer)	677.0518	2.8306	dbspec-13-Mar-2023-10-31-56.088	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
16	Bacillus anthracis unknown origin (A106, Beyer)	676.5292	2.8303	dbspec-13-Mar-2023-10-27-17.235	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus anthracis ATCC 14578 (A71, Beyer)	675.3175	2.8295	dbspec-13-Mar-2023-10-23-57.94	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Bacillus anthracis unknown origin (A107, Beyer)	675.0884	2.8294	dbspec-13-Mar-2023-10-27-22.469	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
19	Bacillus anthracis unknown origin (A38, Beyer)	672.679	2.8278	dbspec-13-Mar-2023-10-30-38.418	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
20	Bacillus anthracis unknown origin (A62, Beyer)	671.9918	2.8274	dbspec-13-Mar-2023-10-31-50.057	RKI ZBS6, Dr. Beyer (Uni Hohenheim)

 
 
 
 

---

   

# 10 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0005'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0005
type:		Measurement 01
NCBI ID (primary):		529
NCBI ID (secondary):		529
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Ochrobactrum anthropi A-148-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:25:30.359+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_05/0_H11/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Ochrobactrum anthropi	863.9247	2.9365	529	2
2	Brucella intermedia	150.8643	2.1786	94625	1
3	Burkholderia plantarii	74.7937	1.8739	41899	2
4	Brucella inopinata	36.5417	1.5628	1218315	1
5	Brucella abortus	13.8185	1.1405	235	16

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Ochrobactrum anthropi DSM 20150, ATCC 11425	741.69	2.8702	dbspec-13-Mar-2023-13-25-52.917	RKI ZBS6
2	Ochrobactrum anthropi Ring trial A-269 08 A	715.2071	2.8544	dbspec-13-Mar-2023-13-25-50.058	RKI ZBS2|ZBS6
3	Brucella intermedia A1309	382.801	2.583	dbspec-13-Mar-2023-11-53-15.664	RKI ZBS6
4	Burkholderia plantarii LMG 9035	243.3548	2.3862	dbspec-13-Mar-2023-12-33-26.031	RKI ZBS2|ZBS6
5	Brucella inopinata 10 1 001 44	230.3043	2.3623	dbspec-13-Mar-2023-11-52-46.337	Dr. Tomaso (FLI Jena)
6	Brucella abortus 1 544 03RB0215 3	225.4067	2.353	dbspec-13-Mar-2023-11-43-30.255	Dr. Tomaso (FLI Jena)
7	Burkholderia plantarii LMG 10907	215.0188	2.3325	dbspec-13-Mar-2023-12-33-22.078	RKI ZBS2|ZBS6
8	Enterococcus faecium UW5909 (vanA)	213.9141	2.3302	dbspec-13-Mar-2023-13-1-30.605	RKI ZBS6
9	Burkholderia cenocepacia LMG 16654	213.0694	2.3285	dbspec-13-Mar-2023-12-11-36.547	RKI ZBS6
10	Brucella abortus NCTC 10503 07RB1200, ATCC 23451	212.4545	2.3273	dbspec-13-Mar-2023-11-46-54.219	Dr. Tomaso (FLI Jena)
11	Enterococcus faecium AK-EM28 (ST22)	211.2114	2.3247	dbspec-13-Mar-2023-13-4-10.695	RKI ZBS6
12	Enterococcus faecium UW1328	211.1488	2.3246	dbspec-13-Mar-2023-13-5-33.256	RKI ZBS6
13	Stenotrophomonas maltophilia Sm 132	205.4251	2.3127	dbspec-13-Mar-2023-13-49-3.824	RKI ZBS6
14	Stenotrophomonas maltophilia Sm 131	201.6704	2.3046	dbspec-13-Mar-2023-13-49-1.715	RKI ZBS6
15	Stenotrophomonas maltophilia Sm 20|06	200.5695	2.3023	dbspec-13-Mar-2023-13-49-5.824	RKI ZBS6
16	Stenotrophomonas maltophilia DSM 50170(T)	199.2529	2.2994	dbspec-13-Mar-2023-13-48-53.981	RKI ZBS6
17	Stenotrophomonas maltophilia Sm 51	199.0626	2.299	dbspec-13-Mar-2023-13-49-16.433	RKI ZBS6
18	Brucella vulpis DSM 101715	197.6521	2.2959	dbspec-13-Mar-2023-12-6-18.116	RKI ZBS6
19	Brucella sp. 83-13	197.1536	2.2948	dbspec-13-Mar-2023-12-2-8.512	RKI FG14
20	Francisella orientalis DSM 21254, LMG 24544	194.8232	2.2896	dbspec-13-Mar-2023-13-9-17.626	RKI ZBS6

 
 
 
 

---

   

# 11 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0005'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0005
type:		Measurement 02
NCBI ID (primary):		529
NCBI ID (secondary):		529
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Ochrobactrum anthropi A-148-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:00:58.531+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_05/0_H12/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Ochrobactrum anthropi	835.4138	2.9219	529	2
2	Brucella intermedia	141.8669	2.1519	94625	1
3	Brucella abortus	49.0663	1.6908	235	16
4	Brucella vulpis	36.8811	1.5668	981386	1
5	Brucella ovis	7.9254	0.89902	236	4

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Ochrobactrum anthropi DSM 20150, ATCC 11425	685.0486	2.8357	dbspec-13-Mar-2023-13-25-52.917	RKI ZBS6
2	Ochrobactrum anthropi Ring trial A-269 08 A	683.8332	2.835	dbspec-13-Mar-2023-13-25-50.058	RKI ZBS2|ZBS6
3	Brucella intermedia A1309	353.3501	2.5482	dbspec-13-Mar-2023-11-53-15.664	RKI ZBS6
4	Brucella abortus 1 544 03RB0215 3	233.6293	2.3685	dbspec-13-Mar-2023-11-43-30.255	Dr. Tomaso (FLI Jena)
5	Brucella vulpis DSM 101715	232.0958	2.3657	dbspec-13-Mar-2023-12-6-18.116	RKI ZBS6
6	Brucella abortus NCTC 10503 07RB1200, ATCC 23451	222.5444	2.3474	dbspec-13-Mar-2023-11-46-54.219	Dr. Tomaso (FLI Jena)
7	Brucella ovis 63 290 03RB0213 3	211.5089	2.3253	dbspec-13-Mar-2023-12-0-31.733	Dr. Tomaso (FLI Jena)
8	Brucella canis R 6 66 03RB0214 3	211.4169	2.3251	dbspec-13-Mar-2023-11-50-20.215	Dr. Tomaso (FLI Jena)
9	Enterococcus faecium UBA8.6	211.4148	2.3251	dbspec-13-Mar-2023-13-5-21.881	RKI ZBS6
10	Ralstonia mannitolilytica DSM 17512, LMG 6866	211.3225	2.3249	dbspec-13-Mar-2023-13-35-28.326	RKI ZBS6
11	Brucella sp. 83-13	210.3665	2.323	dbspec-13-Mar-2023-12-2-8.512	RKI FG14
12	Burkholderia plantarii LMG 9035	206.683	2.3153	dbspec-13-Mar-2023-12-33-26.031	RKI ZBS2|ZBS6
13	Brucella inopinata 10 1 001 44	204.7909	2.3113	dbspec-13-Mar-2023-11-52-46.337	Dr. Tomaso (FLI Jena)
14	Brucella melitensis 02 3 022 17	204.7465	2.3112	dbspec-13-Mar-2023-11-54-33.584	Dr. Tomaso (FLI Jena)
15	Brucella melitensis 02 2 021 14	203.2183	2.308	dbspec-13-Mar-2023-11-54-0.366	Dr. Tomaso (FLI Jena)
16	Brucella canis (BM385)	202.24	2.3059	dbspec-13-Mar-2023-11-49-5.154	TNO Netherlands
17	Brucella abortus 1 EQADeBa 09RB8922	200.8003	2.3028	dbspec-13-Mar-2023-11-43-55.551	Dr. Tomaso (FLI Jena)
18	Brucella abortus 01 1 001 1	199.5683	2.3001	dbspec-13-Mar-2023-11-43-0.256	Dr. Tomaso (FLI Jena)
19	Brucella sp. A 1755_28	199.0689	2.299	dbspec-13-Mar-2023-12-2-58.167	RKI ZBS6
20	Francisella orientalis DSM 21254, LMG 24544	196.5848	2.2935	dbspec-13-Mar-2023-13-9-17.626	RKI ZBS6

 
 
 
 

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# 12 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0006'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0006
type:		Measurement 01
NCBI ID (primary):		633
NCBI ID (secondary):		633
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pseudotuberculosis III: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:36:26.328+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_06/0_H13/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Yersinia pseudotuberculosis	794.5985	2.9001	633	24
2	Yersinia pestis	66.4509	1.8225	632	11
3	Yersinia pestis	0.19175	-0.71725	632.1	8
4	Yersinia similis	0.00011974	-3.9218	367190	2

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Yersinia pseudotuberculosis 29827	796.898	2.9014	dbspec-13-Mar-2023-14-9-22.061	RKI ZBS6
2	Yersinia pseudotuberculosis 29490	719.2671	2.8569	dbspec-13-Mar-2023-14-9-18.155	RKI ZBS6
3	Yersinia pseudotuberculosis 25743	693.0166	2.8407	dbspec-13-Mar-2023-14-8-54.906	RKI ZBS6
4	Yersinia pseudotuberculosis VI	676.1346	2.83	dbspec-13-Mar-2023-14-9-54.107	RKI ZBS6
5	Yersinia pestis NCTC 2028	651.2986	2.8138	dbspec-13-Mar-2023-14-6-48.998	Dr. Drevinek (Sujchbo, Czech Republic)
6	Yersinia pseudotuberculosis DSM 22972	645.9469	2.8102	dbspec-13-Mar-2023-14-9-34.53	RKI ZBS6
7	Yersinia pestis NCTC 10030	636.4149	2.8037	dbspec-13-Mar-2023-14-6-21.467	Dr. Drevinek (Sujchbo, Czech Republic)
8	Yersinia pseudotuberculosis J9	635.133	2.8029	dbspec-13-Mar-2023-14-9-25.936	RKI ZBS6
9	Yersinia pseudotuberculosis 07PW12234	634.6433	2.8025	dbspec-13-Mar-2023-14-8-45.968	RKI ZBS6
10	Yersinia pseudotuberculosis 25858	633.7922	2.8019	dbspec-13-Mar-2023-14-8-58.843	RKI ZBS6
11	Yersinia pseudotuberculosis 27705	633.6207	2.8018	dbspec-13-Mar-2023-14-9-2.624	RKI ZBS6
12	Yersinia pseudotuberculosis 27707	630.2236	2.7995	dbspec-13-Mar-2023-14-9-6.702	RKI ZBS6
13	Yersinia pestis RV 3	626.556	2.797	dbspec-13-Mar-2023-14-8-16.344	RKI ZBS6
14	Yersinia pseudotuberculosis 28928	623.4508	2.7948	dbspec-13-Mar-2023-14-9-14.421	RKI ZBS6
15	Yersinia pestis NCTC 2868	618.8783	2.7916	dbspec-13-Mar-2023-14-7-2.388	Dr. Drevinek (Sujchbo, Czech Republic)
16	Yersinia pestis NCTC 5923, ATCC 19428	607.5134	2.7836	dbspec-13-Mar-2023-14-7-33.278	Dr. Drevinek (Sujchbo, Czech Republic)
17	Yersinia pestis NCTC 570	598.9738	2.7774	dbspec-13-Mar-2023-14-7-15.294	Dr. Drevinek (Sujchbo, Czech Republic)
18	Yersinia similis DSM 18211, LMG 23763, CCUG 52882	598.5899	2.7771	dbspec-13-Mar-2023-14-10-30.841	CVUA Stuttgart
19	Yersinia pseudotuberculosis 06PW40285	592.0495	2.7724	dbspec-13-Mar-2023-14-8-36.75	RKI ZBS6
20	Yersinia pseudotuberculosis DSM 8992, ATCC 29833, NCTC 10275, CIP 55.85	588.056	2.7694	dbspec-13-Mar-2023-14-9-39.326	RKI ZBS6

 
 
 
 

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# 13 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0006'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0006
type:		Measurement 02
NCBI ID (primary):		633
NCBI ID (secondary):		633
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pseudotuberculosis III: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:22:37.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_06/0_H14/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Yersinia pseudotuberculosis	865.5884	2.9373	633	24
2	Yersinia pestis	9.9598	0.99825	632.1	8
3	Yersinia pestis	0.19569	-0.70843	632	11
4	Yersinia similis	1.8785e-08	-7.7262	367190	2

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Yersinia pseudotuberculosis 29827	767.2482	2.8849	dbspec-13-Mar-2023-14-9-22.061	RKI ZBS6
2	Yersinia pseudotuberculosis VI	765.9971	2.8842	dbspec-13-Mar-2023-14-9-54.107	RKI ZBS6
3	Yersinia pseudotuberculosis 29490	746.2318	2.8729	dbspec-13-Mar-2023-14-9-18.155	RKI ZBS6
4	Yersinia pseudotuberculosis 25743	719.6897	2.8571	dbspec-13-Mar-2023-14-8-54.906	RKI ZBS6
5	Yersinia pseudotuberculosis J9	716.6859	2.8553	dbspec-13-Mar-2023-14-9-25.936	RKI ZBS6
6	Yersinia pseudotuberculosis 27707	715.994	2.8549	dbspec-13-Mar-2023-14-9-6.702	RKI ZBS6
7	Yersinia pseudotuberculosis 07PW12234	713.0691	2.8531	dbspec-13-Mar-2023-14-8-45.968	RKI ZBS6
8	Yersinia pestis RV 3	710.1778	2.8514	dbspec-13-Mar-2023-14-8-16.344	RKI ZBS6
9	Yersinia pseudotuberculosis 27705	692.0613	2.8401	dbspec-13-Mar-2023-14-9-2.624	RKI ZBS6
10	Yersinia pseudotuberculosis 25858	688.5772	2.838	dbspec-13-Mar-2023-14-8-58.843	RKI ZBS6
11	Yersinia pseudotuberculosis 06PW40285	673.9136	2.8286	dbspec-13-Mar-2023-14-8-36.75	RKI ZBS6
12	Yersinia pseudotuberculosis Typ3 P- INV+	665.4429	2.8231	dbspec-13-Mar-2023-14-9-50.326	RKI ZBS6
13	Yersinia pestis NCTC 10030	663.7452	2.822	dbspec-13-Mar-2023-14-6-21.467	Dr. Drevinek (Sujchbo, Czech Republic)
14	Yersinia pseudotuberculosis 28928	650.7801	2.8134	dbspec-13-Mar-2023-14-9-14.421	RKI ZBS6
15	Yersinia pseudotuberculosis Ring trial A-269 04 B	648.9662	2.8122	dbspec-13-Mar-2023-14-9-30.061	RKI ZBS2|ZBS6
16	Yersinia pseudotuberculosis DSM 22972	644.9111	2.8095	dbspec-13-Mar-2023-14-9-34.53	RKI ZBS6
17	Yersinia pseudotuberculosis DSM 8992, ATCC 29833, NCTC 10275, CIP 55.85	644.6554	2.8093	dbspec-13-Mar-2023-14-9-39.326	RKI ZBS6
18	Yersinia pseudotuberculosis I	643.2043	2.8083	dbspec-13-Mar-2023-14-9-46.529	RKI ZBS6
19	Yersinia pseudotuberculosis 04PA01423	636.3179	2.8037	dbspec-13-Mar-2023-14-8-17.969	RKI ZBS6
20	Yersinia similis IMB 4354	630.8498	2.7999	dbspec-13-Mar-2023-14-10-51.809	Prof. Fuchs (ZIEL, Munich)

 
 
 
 

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# 14 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0007'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0007
type:		Measurement 01
NCBI ID (primary):		13373
NCBI ID (secondary):		13373
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia mallei A106-3: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:39:43.625+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_07/0_H15/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia mallei	823.1333	2.9155	13373	33
2	Burkholderia pseudomallei	7.5881e-05	-4.1199	28450	20
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia mallei Mukteswar 290103RR0041	699.7849	2.845	dbspec-13-Mar-2023-12-21-5.206	Dr. Tomaso (FLI Jena)
2	Burkholderia mallei 3708	659.6933	2.8193	dbspec-13-Mar-2023-12-27-35.323	RKI ZBS6
3	Burkholderia mallei Dubai 7	650.3409	2.8131	dbspec-13-Mar-2023-12-27-36.994	Dr. Tomaso (FLI Jena)
4	Burkholderia mallei Dubai 7 240609RR5318	643.1621	2.8083	dbspec-13-Mar-2023-12-20-29.769	Dr. Tomaso (FLI Jena)
5	Burkholderia mallei ATCC 23344	632.5265	2.8011	dbspec-13-Mar-2023-12-21-57.596	RKI ZBS6
6	Burkholderia mallei BfR M1 290103RR0043	617.6222	2.7907	dbspec-13-Mar-2023-12-26-31.59	Dr. Tomaso (FLI Jena)
7	Burkholderia mallei ATCC 23344 300102RR0118	617.2581	2.7905	dbspec-13-Mar-2023-12-22-41.157	Dr. Tomaso (FLI Jena)
8	Burkholderia mallei BfR 237 061102RR0551	616.4499	2.7899	dbspec-13-Mar-2023-12-23-35.562	Dr. Tomaso (FLI Jena)
9	Burkholderia mallei ATCC 23344 251109RR8925	615.4124	2.7892	dbspec-13-Mar-2023-12-22-6.064	Dr. Tomaso (FLI Jena)
10	Burkholderia mallei type strain	614.3949	2.7884	dbspec-13-Mar-2023-12-31-17.112	Dr. Tomaso (FLI Jena)
11	Burkholderia mallei NCTC 10260 041206RR1051	614.174	2.7883	dbspec-13-Mar-2023-12-29-19.364	Dr. Tomaso (FLI Jena)
12	Burkholderia mallei BfR 34 281002RR0518	613.5107	2.7878	dbspec-13-Mar-2023-12-25-35.356	Dr. Tomaso (FLI Jena)
13	Burkholderia mallei NCTC 10247 041206RR1056	612.6771	2.7872	dbspec-13-Mar-2023-12-28-54.802	Dr. Tomaso (FLI Jena)
14	Burkholderia mallei Zagreb 080304RR0090	611.8271	2.7866	dbspec-13-Mar-2023-12-21-33.94	Dr. Tomaso (FLI Jena)
15	Burkholderia mallei Bogor 211101RR0419	611.6958	2.7865	dbspec-13-Mar-2023-12-20-7.035	Dr. Tomaso (FLI Jena)
16	Burkholderia mallei BfR M1 040203RR0053	611.517	2.7864	dbspec-13-Mar-2023-12-26-2.246	Dr. Tomaso (FLI Jena)
17	Burkholderia mallei NCTC 120 Lister 041206RR1055	610.9017	2.786	dbspec-13-Mar-2023-12-29-46.989	Dr. Tomaso (FLI Jena)
18	Burkholderia pseudomallei ring trial A-269 01 A	610.1186	2.7854	dbspec-13-Mar-2023-12-33-29.89	RKI ZBS2|ZBS6
19	Burkholderia pseudomallei 11642	597.8363	2.7766	dbspec-13-Mar-2023-12-35-0.476	RKI ZBS6
20	Burkholderia pseudomallei 03 04450 060406RR0740	585.5559	2.7676	dbspec-13-Mar-2023-12-33-58.03	Dr. Tomaso (FLI Jena)

 
 
 
 

---

   

# 15 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0007'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0007
type:		Measurement 02
NCBI ID (primary):		13373
NCBI ID (secondary):		13373
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia mallei A106-3: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:30:51.015+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_07/0_H16/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia mallei	781.4591	2.8929	13373	33
2	Burkholderia pseudomallei	48.9573	1.6898	28450	20
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia mallei Mukteswar 290103RR0041	714.3034	2.8539	dbspec-13-Mar-2023-12-21-5.206	Dr. Tomaso (FLI Jena)
2	Burkholderia mallei Dubai 7	678.1305	2.8313	dbspec-13-Mar-2023-12-27-36.994	Dr. Tomaso (FLI Jena)
3	Burkholderia mallei 3708	657.8465	2.8181	dbspec-13-Mar-2023-12-27-35.323	RKI ZBS6
4	Burkholderia mallei type strain	640.4469	2.8065	dbspec-13-Mar-2023-12-31-17.112	Dr. Tomaso (FLI Jena)
5	Burkholderia pseudomallei ring trial A-269 01 A	636.8895	2.8041	dbspec-13-Mar-2023-12-33-29.89	RKI ZBS2|ZBS6
6	Burkholderia mallei ATCC 23344	635.6783	2.8032	dbspec-13-Mar-2023-12-21-57.596	RKI ZBS6
7	Burkholderia mallei ATCC 23344 300102RR0118	614.6219	2.7886	dbspec-13-Mar-2023-12-22-41.157	Dr. Tomaso (FLI Jena)
8	Burkholderia mallei BfR M1 290103RR0043	614.4376	2.7885	dbspec-13-Mar-2023-12-26-31.59	Dr. Tomaso (FLI Jena)
9	Burkholderia mallei BfR 237 061102RR0551	613.7286	2.788	dbspec-13-Mar-2023-12-23-35.562	Dr. Tomaso (FLI Jena)
10	Burkholderia mallei Dubai 7 240609RR5318	613.582	2.7879	dbspec-13-Mar-2023-12-20-29.769	Dr. Tomaso (FLI Jena)
11	Burkholderia mallei NCTC 10260 041206RR1051	611.235	2.7862	dbspec-13-Mar-2023-12-29-19.364	Dr. Tomaso (FLI Jena)
12	Burkholderia mallei BfR 34 281002RR0518	610.7558	2.7859	dbspec-13-Mar-2023-12-25-35.356	Dr. Tomaso (FLI Jena)
13	Burkholderia mallei NCTC 10247 041206RR1056	610.5564	2.7857	dbspec-13-Mar-2023-12-28-54.802	Dr. Tomaso (FLI Jena)
14	Burkholderia mallei BfR M1 040203RR0053	609.7706	2.7852	dbspec-13-Mar-2023-12-26-2.246	Dr. Tomaso (FLI Jena)
15	Burkholderia mallei Bogor 211101RR0419	609.3547	2.7849	dbspec-13-Mar-2023-12-20-7.035	Dr. Tomaso (FLI Jena)
16	Burkholderia mallei NCTC 120 Lister 041206RR1055	607.9727	2.7839	dbspec-13-Mar-2023-12-29-46.989	Dr. Tomaso (FLI Jena)
17	Burkholderia pseudomallei A333-1	605.2286	2.7819	dbspec-13-Mar-2023-12-34-28.195	RKI ZBS2
18	Burkholderia mallei Bogur	600.3241	2.7784	dbspec-13-Mar-2023-12-27-23.042	RKI ZBS6
19	Burkholderia mallei Zagreb	596.667	2.7757	dbspec-13-Mar-2023-12-30-45.425	RKI ZBS6
20	Burkholderia pseudomallei NCTC 1688 041206RR1062	591.3938	2.7719	dbspec-13-Mar-2023-12-35-39.413	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 16 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0002'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0002
type:		Measurement 01
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 30 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:23:57.843+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_E20/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia thailandensis	816.5874	2.912	57975	15
2	Burkholderia pseudomallei	0.0040263	-2.3951	28450	20
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia thailandensis E163	669.789	2.8259	dbspec-13-Mar-2023-12-43-28.137	RKI ZBS6
2	Burkholderia thailandensis E125	668.1066	2.8248	dbspec-13-Mar-2023-12-43-3.981	RKI ZBS6
3	Burkholderia thailandensis E207	645.5571	2.8099	dbspec-13-Mar-2023-12-43-39.731	RKI ZBS6
4	Burkholderia thailandensis E184	642.2024	2.8077	dbspec-13-Mar-2023-12-43-32.106	RKI ZBS6
5	Burkholderia thailandensis LMG 20219	622.9684	2.7945	dbspec-13-Mar-2023-12-43-43.45	RKI ZBS2|ZBS6
6	Burkholderia thailandensis DSM 13276	620.5623	2.7928	dbspec-13-Mar-2023-12-42-45.45	RKI ZBS6
7	Burkholderia thailandensis E131	616.3726	2.7898	dbspec-13-Mar-2023-12-43-15.778	RKI ZBS6
8	Burkholderia thailandensis E143	615.131	2.789	dbspec-13-Mar-2023-12-43-19.996	RKI ZBS6
9	Burkholderia thailandensis E067	613.8937	2.7881	dbspec-13-Mar-2023-12-43-0.059	RKI ZBS6
10	Burkholderia thailandensis E058	612.0542	2.7868	dbspec-13-Mar-2023-12-42-56.184	RKI ZBS6
11	Burkholderia thailandensis ring trial A-269 07 A	606.2118	2.7826	dbspec-13-Mar-2023-12-42-43.356	RKI ZBS2|ZBS6
12	Burkholderia thailandensis E202	584.382	2.7667	dbspec-13-Mar-2023-12-43-35.934	RKI ZBS6
13	Burkholderia thailandensis E153	552.9182	2.7427	dbspec-13-Mar-2023-12-43-24.09	RKI ZBS6
14	Burkholderia thailandensis E049	552.1926	2.7421	dbspec-13-Mar-2023-12-42-52.075	RKI ZBS6
15	Burkholderia thailandensis 090804RR0288 135	457.2344	2.6601	dbspec-13-Mar-2023-12-42-20.435	Dr. Tomaso (FLI Jena)
16	Burkholderia pseudomallei A335-1	411.7871	2.6147	dbspec-13-Mar-2023-12-34-35.273	RKI ZBS2
17	Burkholderia pseudomallei CCUG 15649	405.2283	2.6077	dbspec-13-Mar-2023-12-34-50.132	RKI ZBS6
18	Burkholderia pseudomallei CCUG 13790	398.4239	2.6003	dbspec-13-Mar-2023-12-34-42.226	RKI ZBS6
19	Burkholderia pseudomallei CCUG 15651	371.5325	2.57	dbspec-13-Mar-2023-12-34-54.445	RKI ZBS6
20	Burkholderia pseudomallei 4845	358.2464	2.5542	dbspec-13-Mar-2023-12-35-5.554	RKI ZBS6

 
 
 
 

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# 17 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0008'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0008
type:		Measurement 02
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:48:34.328+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_H17/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia thailandensis	880.3244	2.9446	57975	15
2	Burkholderia mallei	0.0042845	-2.3681	13373	33
3	n/a	0	-Inf

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia thailandensis DSM 13276	768.1893	2.8855	dbspec-13-Mar-2023-12-42-45.45	RKI ZBS6
2	Burkholderia thailandensis E202	765.5871	2.884	dbspec-13-Mar-2023-12-43-35.934	RKI ZBS6
3	Burkholderia thailandensis E184	764.1712	2.8832	dbspec-13-Mar-2023-12-43-32.106	RKI ZBS6
4	Burkholderia thailandensis E125	737.7671	2.8679	dbspec-13-Mar-2023-12-43-3.981	RKI ZBS6
5	Burkholderia thailandensis E207	737.1269	2.8675	dbspec-13-Mar-2023-12-43-39.731	RKI ZBS6
6	Burkholderia thailandensis E131	735.7045	2.8667	dbspec-13-Mar-2023-12-43-15.778	RKI ZBS6
7	Burkholderia thailandensis E153	735.4159	2.8665	dbspec-13-Mar-2023-12-43-24.09	RKI ZBS6
8	Burkholderia thailandensis E058	733.3489	2.8653	dbspec-13-Mar-2023-12-42-56.184	RKI ZBS6
9	Burkholderia thailandensis E143	733.3423	2.8653	dbspec-13-Mar-2023-12-43-19.996	RKI ZBS6
10	Burkholderia thailandensis E049	732.7344	2.8649	dbspec-13-Mar-2023-12-42-52.075	RKI ZBS6
11	Burkholderia thailandensis E067	707.8251	2.8499	dbspec-13-Mar-2023-12-43-0.059	RKI ZBS6
12	Burkholderia thailandensis E163	707.4733	2.8497	dbspec-13-Mar-2023-12-43-28.137	RKI ZBS6
13	Burkholderia thailandensis ring trial A-269 07 A	614.5637	2.7886	dbspec-13-Mar-2023-12-42-43.356	RKI ZBS2|ZBS6
14	Burkholderia thailandensis 090804RR0288 135	560.3891	2.7485	dbspec-13-Mar-2023-12-42-20.435	Dr. Tomaso (FLI Jena)
15	Burkholderia thailandensis LMG 20219	541.1567	2.7333	dbspec-13-Mar-2023-12-43-43.45	RKI ZBS2|ZBS6
16	Burkholderia mallei BfR 242 041206RR1050	449.6762	2.6529	dbspec-13-Mar-2023-12-24-4.577	Dr. Tomaso (FLI Jena)
17	Burkholderia mallei BfR 242 300102RR0106	449.0297	2.6523	dbspec-13-Mar-2023-12-24-37.295	Dr. Tomaso (FLI Jena)
18	Burkholderia mallei BfR 235 300102RR0104	448.5281	2.6518	dbspec-13-Mar-2023-12-23-8	Dr. Tomaso (FLI Jena)
19	Burkholderia mallei BfR M2 041206RR1057	447.2179	2.6505	dbspec-13-Mar-2023-12-26-53.324	Dr. Tomaso (FLI Jena)
20	Burkholderia mallei NCTC 10247 041206RR1056	446.4352	2.6498	dbspec-13-Mar-2023-12-28-54.802	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 18 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0008'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0008
type:		Measurement 03
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:36:55.281+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_H18/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Burkholderia thailandensis	872.6275	2.9408	57975	15
2	Burkholderia pseudomallei	0.0036705	-2.4353	28450	20
3	Burkholderia mallei	0.00060161	-3.2207	13373	33

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Burkholderia thailandensis DSM 13276	767.1646	2.8849	dbspec-13-Mar-2023-12-42-45.45	RKI ZBS6
2	Burkholderia thailandensis E125	737.1839	2.8676	dbspec-13-Mar-2023-12-43-3.981	RKI ZBS6
3	Burkholderia thailandensis E067	736.2407	2.867	dbspec-13-Mar-2023-12-43-0.059	RKI ZBS6
4	Burkholderia thailandensis E184	735.5702	2.8666	dbspec-13-Mar-2023-12-43-32.106	RKI ZBS6
5	Burkholderia thailandensis E163	734.939	2.8663	dbspec-13-Mar-2023-12-43-28.137	RKI ZBS6
6	Burkholderia thailandensis E143	732.6363	2.8649	dbspec-13-Mar-2023-12-43-19.996	RKI ZBS6
7	Burkholderia thailandensis E207	706.2566	2.849	dbspec-13-Mar-2023-12-43-39.731	RKI ZBS6
8	Burkholderia thailandensis E131	705.3297	2.8484	dbspec-13-Mar-2023-12-43-15.778	RKI ZBS6
9	Burkholderia thailandensis E202	703.6123	2.8473	dbspec-13-Mar-2023-12-43-35.934	RKI ZBS6
10	Burkholderia thailandensis E058	703.2393	2.8471	dbspec-13-Mar-2023-12-42-56.184	RKI ZBS6
11	Burkholderia thailandensis E049	703.1183	2.847	dbspec-13-Mar-2023-12-42-52.075	RKI ZBS6
12	Burkholderia thailandensis E153	677.2572	2.8308	dbspec-13-Mar-2023-12-43-24.09	RKI ZBS6
13	Burkholderia thailandensis ring trial A-269 07 A	642.7946	2.8081	dbspec-13-Mar-2023-12-42-43.356	RKI ZBS2|ZBS6
14	Burkholderia thailandensis LMG 20219	622.9881	2.7945	dbspec-13-Mar-2023-12-43-43.45	RKI ZBS2|ZBS6
15	Burkholderia thailandensis 090804RR0288 135	524.9824	2.7201	dbspec-13-Mar-2023-12-42-20.435	Dr. Tomaso (FLI Jena)
16	Burkholderia pseudomallei PITT 5691 041206RR1064	448.6769	2.6519	dbspec-13-Mar-2023-12-36-28.661	Dr. Tomaso (FLI Jena)
17	Burkholderia mallei BfR M2 041206RR1057	444.1621	2.6475	dbspec-13-Mar-2023-12-26-53.324	Dr. Tomaso (FLI Jena)
18	Burkholderia pseudomallei CCUG 15649	421.769	2.6251	dbspec-13-Mar-2023-12-34-50.132	RKI ZBS6
19	Burkholderia pseudomallei PITT 521 041206RR1059	419.8068	2.623	dbspec-13-Mar-2023-12-36-9.146	Dr. Tomaso (FLI Jena)
20	Burkholderia mallei Zagreb	418.5418	2.6217	dbspec-13-Mar-2023-12-30-19.066	Dr. Tomaso (FLI Jena)

 
 
 
 

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# 19 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0003'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0003
type:		Measurement 01
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 30 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:29:00.578+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_E21/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Yersinia pestis	385.6608	2.5862	632	11
2	Yersinia similis	55.8603	1.7471	367190	2
3	Bacillus anthracis	43.014	1.6336	1392	130
4	Vibrio cholerae	21.2864	1.3281	666	52
5	Yersinia pseudotuberculosis	1.1028	0.042477	633	24

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Yersinia pestis O3-01500	350.5994	2.5448	dbspec-13-Mar-2023-14-7-44.626	RKI ZBS6
2	Yersinia pestis O3-01501	302.3224	2.4805	dbspec-13-Mar-2023-14-7-48.532	RKI ZBS6
3	Yersinia pestis CCUG EV 76, CCUG 32133	288.5371	2.4602	dbspec-13-Mar-2023-14-6-7.546	RKI ZBS6
4	Bacillus anthracis unknown origin (A122, Beyer)	272.2389	2.435	dbspec-13-Mar-2023-10-28-32.483	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
5	Yersinia similis IMB 4354	264.3254	2.4221	dbspec-13-Mar-2023-14-10-51.809	Prof. Fuchs (ZIEL, Munich)
6	Vibrio cholerae 189-1	252.3681	2.402	dbspec-13-Mar-2023-13-53-59.599	RKI ZBS2|ZBS6
7	Yersinia similis DSM 18211, LMG 23763, CCUG 52882	241.7081	2.3833	dbspec-13-Mar-2023-14-10-30.841	CVUA Stuttgart
8	Vibrio cholerae A482-1	239.6772	2.3796	dbspec-13-Mar-2023-13-56-41.58	RKI ZBS6
9	Vibrio cholerae A482-2	237.4842	2.3756	dbspec-13-Mar-2023-13-56-46.173	RKI ZBS6
10	Yersinia pseudotuberculosis 07PW12234	225.6598	2.3535	dbspec-13-Mar-2023-14-8-45.968	RKI ZBS6
11	Yersinia pestis O3-01504	225.5003	2.3531	dbspec-13-Mar-2023-14-7-59.516	RKI ZBS6
12	Yersinia pestis O3-01502	224.3157	2.3509	dbspec-13-Mar-2023-14-7-52.251	RKI ZBS6
13	Alkalihalobacillus halodurans DSM 6940	223.7891	2.3498	dbspec-13-Mar-2023-10-18-49.043	RKI ZBS6
14	Yersinia pseudotuberculosis Typ3 P- INV+	222.3006	2.3469	dbspec-13-Mar-2023-14-9-50.326	RKI ZBS6
15	Alkalihalobacillus halodurans DSM 497	221.8675	2.3461	dbspec-13-Mar-2023-10-18-18.499	RKI ZBS6
16	Bacillus anthracis animal (A40, Beyer)	221.5854	2.3455	dbspec-13-Mar-2023-10-25-53.362	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Yersinia pseudotuberculosis 04PA01423	221.4696	2.3453	dbspec-13-Mar-2023-14-8-17.969	RKI ZBS6
18	Yersinia pestis NCTC 5923, ATCC 19428	219.7146	2.3419	dbspec-13-Mar-2023-14-7-33.278	Dr. Drevinek (Sujchbo, Czech Republic)
19	Yersinia pseudotuberculosis J9	216.64	2.3357	dbspec-13-Mar-2023-14-9-25.936	RKI ZBS6
20	Yersinia enterocolitica Ring trial A-269 06 B	216.4919	2.3354	dbspec-13-Mar-2023-14-0-26.224	RKI ZBS2|ZBS6

 
 
 
 

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# 20 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0009'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0009
type:		Measurement 02
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 15 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:57:12.250+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_H19/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Yersinia pestis	399.9811	2.602	632	11
2	Bacillus anthracis	80.792	1.9074	1392	130
3	Yersinia similis	48.6004	1.6866	367190	2
4	Yersinia pseudotuberculosis	16.2747	1.2115	633	24
5	Yersinia pestis	2.723	0.43504	632.1	8

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Yersinia pestis O3-01501	368.7671	2.5668	dbspec-13-Mar-2023-14-7-48.532	RKI ZBS6
2	Yersinia pestis O3-01500	334.9428	2.525	dbspec-13-Mar-2023-14-7-44.626	RKI ZBS6
3	Bacillus anthracis unknown origin (A122, Beyer)	328.9738	2.5172	dbspec-13-Mar-2023-10-28-32.483	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
4	Yersinia pestis CCUG EV 76, CCUG 32133	326.7558	2.5142	dbspec-13-Mar-2023-14-6-7.546	RKI ZBS6
5	Yersinia similis IMB 4354	296.393	2.4719	dbspec-13-Mar-2023-14-10-51.809	Prof. Fuchs (ZIEL, Munich)
6	Yersinia pestis NCTC 5923, ATCC 19428	287.6569	2.4589	dbspec-13-Mar-2023-14-7-33.278	Dr. Drevinek (Sujchbo, Czech Republic)
7	Yersinia pseudotuberculosis Typ3 P- INV+	282.6815	2.4513	dbspec-13-Mar-2023-14-9-50.326	RKI ZBS6
8	Yersinia pseudotuberculosis 04PA01423	276.7564	2.4421	dbspec-13-Mar-2023-14-8-17.969	RKI ZBS6
9	Yersinia pestis O3-01502	276.4182	2.4416	dbspec-13-Mar-2023-14-7-52.251	RKI ZBS6
10	Yersinia pseudotuberculosis 27705	270.2117	2.4317	dbspec-13-Mar-2023-14-9-2.624	RKI ZBS6
11	Yersinia similis DSM 18211, LMG 23763, CCUG 52882	268.6606	2.4292	dbspec-13-Mar-2023-14-10-30.841	CVUA Stuttgart
12	Yersinia pseudotuberculosis 07WI00989	267.6929	2.4276	dbspec-13-Mar-2023-14-8-51.031	RKI ZBS6
13	Yersinia pseudotuberculosis 25743	267.6561	2.4276	dbspec-13-Mar-2023-14-8-54.906	RKI ZBS6
14	Yersinia pseudotuberculosis Ring trial A-269 04 B	263.6661	2.4211	dbspec-13-Mar-2023-14-9-30.061	RKI ZBS2|ZBS6
15	Yersinia pseudotuberculosis DSM 22972	251.9859	2.4014	dbspec-13-Mar-2023-14-9-34.53	RKI ZBS6
16	Yersinia pseudotuberculosis 07PW12234	251.9022	2.4012	dbspec-13-Mar-2023-14-8-45.968	RKI ZBS6
17	Bacillus anthracis Sterne vaccine (A123, Beyer)	251.6496	2.4008	dbspec-13-Mar-2023-10-24-59.298	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Yersinia pestis O3-01504	249.2561	2.3966	dbspec-13-Mar-2023-14-7-59.516	RKI ZBS6
19	Yersinia enterocolitica Ring trial A-269 06 B	247.3726	2.3934	dbspec-13-Mar-2023-14-0-26.224	RKI ZBS2|ZBS6
20	Yersinia pestis NCTC 570	245.4798	2.39	dbspec-13-Mar-2023-14-7-15.294	Dr. Drevinek (Sujchbo, Czech Republic)

 
 
 
 

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# 21 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0009'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0009
type:		Measurement 03
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 15 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:44:17.781+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_H20/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Yersinia pestis	385.0124	2.5855	632	11
2	Bacillus anthracis	80.8769	1.9078	1392	130
3	Yersinia similis	78.1345	1.8928	367190	2
4	Yersinia pseudotuberculosis	10.7349	1.0308	633	24
5	Yersinia pestis	2.7896	0.44554	632.1	8

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Yersinia pestis O3-01501	364.5943	2.5618	dbspec-13-Mar-2023-14-7-48.532	RKI ZBS6
2	Yersinia pestis O3-01500	329.6823	2.5181	dbspec-13-Mar-2023-14-7-44.626	RKI ZBS6
3	Bacillus anthracis unknown origin (A122, Beyer)	329.3877	2.5177	dbspec-13-Mar-2023-10-28-32.483	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
4	Yersinia pestis CCUG EV 76, CCUG 32133	325.9052	2.5131	dbspec-13-Mar-2023-14-6-7.546	RKI ZBS6
5	Yersinia similis DSM 18211, LMG 23763, CCUG 52882	306.4703	2.4864	dbspec-13-Mar-2023-14-10-30.841	CVUA Stuttgart
6	Yersinia similis IMB 4354	304.1836	2.4831	dbspec-13-Mar-2023-14-10-51.809	Prof. Fuchs (ZIEL, Munich)
7	Yersinia pseudotuberculosis Typ3 P- INV+	289.9502	2.4623	dbspec-13-Mar-2023-14-9-50.326	RKI ZBS6
8	Yersinia pestis NCTC 5923, ATCC 19428	286.0528	2.4564	dbspec-13-Mar-2023-14-7-33.278	Dr. Drevinek (Sujchbo, Czech Republic)
9	Yersinia pestis O3-01502	283.3844	2.4524	dbspec-13-Mar-2023-14-7-52.251	RKI ZBS6
10	Yersinia enterocolitica Ring trial A-269 06 B	282.4484	2.4509	dbspec-13-Mar-2023-14-0-26.224	RKI ZBS2|ZBS6
11	Yersinia pseudotuberculosis 04PA01423	282.1283	2.4504	dbspec-13-Mar-2023-14-8-17.969	RKI ZBS6
12	Yersinia pseudotuberculosis 27705	277.7353	2.4436	dbspec-13-Mar-2023-14-9-2.624	RKI ZBS6
13	Yersinia pseudotuberculosis 25743	275.3895	2.4399	dbspec-13-Mar-2023-14-8-54.906	RKI ZBS6
14	Yersinia pseudotuberculosis 07WI00989	273.3318	2.4367	dbspec-13-Mar-2023-14-8-51.031	RKI ZBS6
15	Yersinia pseudotuberculosis Ring trial A-269 04 B	270.6463	2.4324	dbspec-13-Mar-2023-14-9-30.061	RKI ZBS2|ZBS6
16	Yersinia pseudotuberculosis 07PW12234	260.1464	2.4152	dbspec-13-Mar-2023-14-8-45.968	RKI ZBS6
17	Yersinia pseudotuberculosis DSM 22972	258.5891	2.4126	dbspec-13-Mar-2023-14-9-34.53	RKI ZBS6
18	Yersinia pestis O3-01504	255.9371	2.4081	dbspec-13-Mar-2023-14-7-59.516	RKI ZBS6
19	Yersinia pseudotuberculosis J9	251.8718	2.4012	dbspec-13-Mar-2023-14-9-25.936	RKI ZBS6
20	Bacillus anthracis animal (A40, Beyer)	251.546	2.4006	dbspec-13-Mar-2023-10-25-53.362	RKI ZBS6, Dr. Beyer (Uni Hohenheim)

 
 
 
 

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# 22 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0004'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0004
type:		Measurement 01
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 80 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:39:08.984+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_E22/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus thuringiensis	707.9331	2.85	1428	20
2	Bacillus cereus	11.4704	1.0596	1396	102
3	Bacillus anthracis	1.5188	0.18151	1392	130

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus thuringiensis DSM 2046 (B188, Beyer)	552.3917	2.7422	dbspec-13-Mar-2023-10-55-6.678	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
2	Bacillus thuringiensis DSM 2046	526.6426	2.7215	dbspec-13-Mar-2023-10-55-12.506	RKI ZBS6
3	Bacillus thuringiensis DSM 6890	515.5886	2.7123	dbspec-13-Mar-2023-10-56-7.598	RKI ZBS6
4	Bacillus thuringiensis WS 2621	504.5297	2.7029	dbspec-13-Mar-2023-10-57-44.127	RKI ZBS6, Prof. Ehling-Schulz
5	Bacillus thuringiensis DSM 350	499.8741	2.6989	dbspec-13-Mar-2023-10-55-19.49	RKI ZBS6
6	Bacillus thuringiensis DSM 5815	499.3059	2.6984	dbspec-13-Mar-2023-10-55-28.505	RKI ZBS6
7	Bacillus thuringiensis DSM 2046 (WS 2734)	476.3955	2.678	dbspec-13-Mar-2023-10-57-47.049	RKI ZBS6, Prof. Ehling-Schulz
8	Bacillus cereus DSM 31 ATCC 14579 (B69, Beyer)	446.3473	2.6497	dbspec-13-Mar-2023-10-38-30.876	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
9	Bacillus cereus DSM 31 (B181, Beyer)	445.9232	2.6493	dbspec-13-Mar-2023-10-38-12.908	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
10	Bacillus thuringiensis DSM 350 (WIS-St. Nr. 315)	440.3233	2.6438	dbspec-13-Mar-2023-10-57-41.268	RKI ZBS6 |WIS (Dr. Niederwoehrmeier, Munster)
11	Bacillus anthracis unknown origin (A9, Beyer)	435.556	2.639	dbspec-13-Mar-2023-10-33-14.227	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
12	Bacillus anthracis unknown origin (A28, Beyer)	435.2244	2.6387	dbspec-13-Mar-2023-10-29-51.2	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis unknown origin (A10, Beyer)	434.627	2.6381	dbspec-13-Mar-2023-10-26-50.642	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus anthracis unknown origin (A63, Beyer)	433.7381	2.6372	dbspec-13-Mar-2023-10-31-56.088	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
15	Bacillus anthracis A300-2b (RKI)	432.8288	2.6363	dbspec-13-Mar-2023-10-21-55.739	RKI ZBS2
16	Bacillus anthracis unknown origin (A73, Beyer)	431.5667	2.635	dbspec-13-Mar-2023-10-32-23.963	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus anthracis unknown origin (A113, Beyer)	430.4868	2.634	dbspec-13-Mar-2023-10-28-10.734	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Bacillus anthracis unknown origin (A32, Beyer)	430.4723	2.6339	dbspec-13-Mar-2023-10-30-8.372	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
19	Bacillus cereus DSM 31 (RKI)	426.4628	2.6299	dbspec-13-Mar-2023-10-38-18.205	RKI ZBS6
20	Bacillus anthracis ATCC 14578 (A71, Beyer)	425.345	2.6287	dbspec-13-Mar-2023-10-23-57.94	RKI ZBS6, Dr. Beyer (Uni Hohenheim)

 
 
 
 

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# 23 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0010'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0010
type:		Measurement 02
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T15:05:30.796+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_H21/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus thuringiensis	804.4046	2.9055	1428	20
2	Bacillus toyonensis	8.2128	0.91449	155322	16
3	Bacillus anthracis	2.307	0.36304	1392	130
4	Bacillus cereus	0.41494	-0.38201	1396	102
5	Bacillus wiedmannii	9.2959e-09	-8.0317	1890302	8

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus thuringiensis DSM 6890	703.6466	2.8474	dbspec-13-Mar-2023-10-56-7.598	RKI ZBS6
2	Bacillus thuringiensis DSM 350	644.8777	2.8095	dbspec-13-Mar-2023-10-55-19.49	RKI ZBS6
3	Bacillus thuringiensis DSM 2046 (B188, Beyer)	641.5966	2.8073	dbspec-13-Mar-2023-10-55-6.678	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
4	Bacillus thuringiensis DSM 5815	617.206	2.7904	dbspec-13-Mar-2023-10-55-28.505	RKI ZBS6
5	Bacillus thuringiensis DSM 2046	615.4634	2.7892	dbspec-13-Mar-2023-10-55-12.506	RKI ZBS6
6	Bacillus thuringiensis DSM 2046 (WS 2734)	603.2017	2.7805	dbspec-13-Mar-2023-10-57-47.049	RKI ZBS6, Prof. Ehling-Schulz
7	Bacillus thuringiensis WS 2621	578.4607	2.7623	dbspec-13-Mar-2023-10-57-44.127	RKI ZBS6, Prof. Ehling-Schulz
8	Bacillus toyonensis NMTD92	513.1037	2.7102	dbspec-13-Mar-2023-10-58-56.219	RKI ZBS6 |Prof. Gao (Nanjing, China)
9	Bacillus thuringiensis NMTD81	511.3273	2.7087	dbspec-13-Mar-2023-10-57-24.862	RKI ZBS6 |Prof. Gao (Nanjing, China)
10	Bacillus anthracis unknown origin (A36, Beyer)	501.2274	2.7	dbspec-13-Mar-2023-10-30-31.199	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
11	Bacillus thuringiensis GBSC29 (02)	500.7105	2.6996	dbspec-13-Mar-2023-10-56-50.363	RKI ZBS6 |Prof. Gao (Nanjing, China)
12	Bacillus cereus DSM 31 (B181, Beyer)	477.4904	2.679	dbspec-13-Mar-2023-10-38-12.908	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis unknown origin (A5, Beyer)	470.6633	2.6727	dbspec-13-Mar-2023-10-31-22.823	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus thuringiensis DSM 350 (WIS-St. Nr. 315)	470.4183	2.6725	dbspec-13-Mar-2023-10-57-41.268	RKI ZBS6 |WIS (Dr. Niederwoehrmeier, Munster)
15	Bacillus anthracis unknown origin (A10, Beyer)	466.9592	2.6693	dbspec-13-Mar-2023-10-26-50.642	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
16	Bacillus anthracis unknown origin, blue (A88, Beyer)	465.8937	2.6683	dbspec-13-Mar-2023-10-33-35.805	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus cereus DSM 31 (RKI)	457.5174	2.6604	dbspec-13-Mar-2023-10-38-18.205	RKI ZBS6
18	Bacillus anthracis Sterne vaccine (A118, Beyer)	456.4808	2.6594	dbspec-13-Mar-2023-10-24-54.204	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
19	Bacillus anthracis unknown origin (A62, Beyer)	452.5967	2.6557	dbspec-13-Mar-2023-10-31-50.057	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
20	Bacillus wiedmannii NMSW23	444.8285	2.6482	dbspec-13-Mar-2023-11-2-54.98	RKI ZBS6 |Prof. Gao (Nanjing, China)

 
 
 
 

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# 24 - IDENTIFICATION REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0010'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0010
type:		Measurement 03
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:51:48.343+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_H22/1/1SLin

 
 
 
 

Identification results: analysis of score ranking list
No.	Genus/Species	Score	Log Score	UniProt Identifier	No. of strains in DB
1	Bacillus thuringiensis	712.1458	2.8526	1428	20
2	Bacillus anthracis	13.917	1.1435	1392	130
3	Bacillus toyonensis	3.7796	0.57745	155322	16
4	Bacillus wiedmannii	5.4428e-05	-4.2642	1890302	8

 
 
 
 

Score ranking list: best matches with test spectrum
No.	Genus/Species/Strain	Score	Log Score	Spectrum identifier	Customer, or UniprotKB link
1	Bacillus thuringiensis DSM 350	581.9254	2.7649	dbspec-13-Mar-2023-10-55-19.49	RKI ZBS6
2	Bacillus thuringiensis DSM 6890	547.8111	2.7386	dbspec-13-Mar-2023-10-56-7.598	RKI ZBS6
3	Bacillus thuringiensis WS 2621	510.7917	2.7082	dbspec-13-Mar-2023-10-57-44.127	RKI ZBS6, Prof. Ehling-Schulz
4	Bacillus thuringiensis DSM 2046 (B188, Beyer)	507.3118	2.7053	dbspec-13-Mar-2023-10-55-6.678	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
5	Bacillus thuringiensis DSM 2046	483.4156	2.6843	dbspec-13-Mar-2023-10-55-12.506	RKI ZBS6
6	Bacillus thuringiensis DSM 2046 (WS 2734)	475.0981	2.6768	dbspec-13-Mar-2023-10-57-47.049	RKI ZBS6, Prof. Ehling-Schulz
7	Bacillus anthracis unknown origin (A36, Beyer)	434.3859	2.6379	dbspec-13-Mar-2023-10-30-31.199	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
8	Bacillus thuringiensis DSM 5815	432.1404	2.6356	dbspec-13-Mar-2023-10-55-28.505	RKI ZBS6
9	Bacillus toyonensis NMTD92	416.9318	2.6201	dbspec-13-Mar-2023-10-58-56.219	RKI ZBS6 |Prof. Gao (Nanjing, China)
10	Bacillus thuringiensis NMTD81	415.388	2.6185	dbspec-13-Mar-2023-10-57-24.862	RKI ZBS6 |Prof. Gao (Nanjing, China)
11	Bacillus thuringiensis GBSC29 (02)	404.7973	2.6072	dbspec-13-Mar-2023-10-56-50.363	RKI ZBS6 |Prof. Gao (Nanjing, China)
12	Bacillus anthracis unknown origin (A10, Beyer)	395.3841	2.597	dbspec-13-Mar-2023-10-26-50.642	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
13	Bacillus anthracis unknown origin (A73, Beyer)	394.6925	2.5963	dbspec-13-Mar-2023-10-32-23.963	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
14	Bacillus anthracis unknown origin, blue (A88, Beyer)	389.6381	2.5907	dbspec-13-Mar-2023-10-33-35.805	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
15	Bacillus anthracis unknown origin (A93, Beyer)	381.0291	2.581	dbspec-13-Mar-2023-10-33-19.711	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
16	Bacillus anthracis Sterne vaccine (A118, Beyer)	379.95	2.5797	dbspec-13-Mar-2023-10-24-54.204	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
17	Bacillus anthracis unknown origin (A62, Beyer)	379.8761	2.5796	dbspec-13-Mar-2023-10-31-50.057	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
18	Bacillus wiedmannii NMSW23	378.8359	2.5785	dbspec-13-Mar-2023-11-2-54.98	RKI ZBS6 |Prof. Gao (Nanjing, China)
19	Bacillus anthracis unknown origin (A5, Beyer)	373.8151	2.5727	dbspec-13-Mar-2023-10-31-22.823	RKI ZBS6, Dr. Beyer (Uni Hohenheim)
20	Bacillus thuringiensis DSM 350 (WIS-St. Nr. 315)	371.2893	2.5697	dbspec-13-Mar-2023-10-57-41.268	RKI ZBS6 |WIS (Dr. Niederwoehrmeier, Munster)

 
 
 
 

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