MicrobeMS QUALITY TEST REPORT OF MICROORGANISM MASS SPECTRA - OVERVIEW  

 

Quality test parameters
number of spectra tested:		24
sequence of preprocessing steps:		AsLS → normalization → peak detection
type of baseline correction:		baseline correction by asymmetric least squares (AsLS)
asymmetry factor (AsLS):		0.0001
lambda factor (AsLS):		100000000
number of iterations (AsLS):		8
type of normalization:		offset correction and vector-norm of mass spectra such that their standard deviation finally equals 1000
parameters of peak detection:		[2000 13000 300 12000 7000 0.075 7.5 800 1 40]
peak search m/z region start value:		2000
peak search m/z region end value:		13000
delta m/z, parameter of the intensity threshold function:		7000
delta counts, parameter of the intensity threshold function:		0.075
slope, parameter of the intensity threshold function:		7.5
resolution of the m/z vector (relative units):		800
fix number of peaks (boolean):		1
number of peaks per spectrum:		40
number of bins of quality test parameters:		10
edges of noise bins:		[20 32 50 80 126 201 318 504 800]
quality parameter 'noise level' - weighting factor:		0.25
edges of baseline error bins:		[150 200 267 356 474 633 844 1125 1500]
quality parameter 'baseline level' - weighting factor:		0.2
edges of bins 'number of peaks':		[5 7 10 14 20 28 40 57 80]
quality parameter 'number of peaks' - weighting factor:		0.3
edges of resolving power bins:		[200 238 283 336 400 476 566 673 800]
quality parameter 'resolving power level' - weighting factor:		0.25
general qt test criteria ('red,'yellow','green'):		[30 45]

 
 
 
 

Short quality test report for MALDI-ToF mass spectra
ID's of test spectra	Noise test result	Baseline test result	'Number of peaks' test result	resolving power test result	Overall quality test result
# 1 - 2013_06_13_Maren_Stämmler_0001     RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains ...	66.7	88.9	55.6	77.8	70.6
# 2 - 2013_06_14_Maren_Stämmler_0001     RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains ...	66.7	100	44.4	77.8	69.4
# 3 - 2013_06_13_Maren_Stämmler_0002     RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains ...	77.8	100	44.4	77.8	72.2
# 4 - 2013_06_14_Maren_Stämmler_0002     RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains ...	55.6	77.8	33.3	66.7	56.1
# 5 - 2013_06_13_Maren_Stämmler_0003     RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains ...	77.8	100	33.3	66.7	66.1
# 6 - 2013_06_14_Maren_Stämmler_0003     RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains ...	66.7	77.8	33.3	66.7	58.9
# 7 - 2013_06_17_Maren_Stämmler_0001     RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains ...	44.4	11.1	55.6	55.6	43.9
# 8 - 2013_06_14_Maren_Stämmler_0004     RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains ...	33.3	11.1	33.3	33.3	28.9
# 9 - 2013_06_13_Maren_Stämmler_0004     RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains ...	44.4	11.1	55.6	55.6	43.9
# 10 - 2013_06_13_Maren_Stämmler_0005     RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains ...	66.7	77.8	55.6	66.7	65.6
# 11 - 2013_06_14_Maren_Stämmler_0005     RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains ...	55.6	33.3	55.6	66.7	53.9
# 12 - 2013_06_13_Maren_Stämmler_0006     RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains ...	66.7	88.9	55.6	77.8	70.6
# 13 - 2013_06_14_Maren_Stämmler_0006     RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains ...	55.6	55.6	55.6	77.8	61.1
# 14 - 2013_06_13_Maren_Stämmler_0007     RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains ...	66.7	100	44.4	77.8	69.4
# 15 - 2013_06_14_Maren_Stämmler_0007     RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains ...	66.7	100	44.4	77.8	69.4
# 16 - 2013_06_17_Maren_Stämmler_0002     RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains ...	44.4	44.4	44.4	55.6	47.2
# 17 - 2013_06_13_Maren_Stämmler_0008     RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains ...	55.6	77.8	55.6	66.7	62.8
# 18 - 2013_06_14_Maren_Stämmler_0008     RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains ...	44.4	33.3	55.6	66.7	51.1
# 19 - 2013_06_17_Maren_Stämmler_0003     RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains ...	77.8	33.3	22.2	33.3	41.1
# 20 - 2013_06_13_Maren_Stämmler_0009     RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains ...	77.8	77.8	22.2	55.6	55.6
# 21 - 2013_06_14_Maren_Stämmler_0009     RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains ...	77.8	55.6	11.1	55.6	47.8
# 22 - 2013_06_17_Maren_Stämmler_0004     RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains ...	33.3	11.1	44.4	44.4	35
# 23 - 2013_06_13_Maren_Stämmler_0010     RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains ...	33.3	11.1	44.4	55.6	37.8
# 24 - 2013_06_14_Maren_Stämmler_0010     RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains ...	33.3	11.1	33.3	33.3	28.9

 
 
 
 

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# 1 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0001'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0001
type:		Measurement 01
NCBI ID (primary):		28450
NCBI ID (secondary):		28450
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia pseudomallei A101-10: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T13:52:45.062+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_01/0_H3/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	71.9539	165.7356	21	589.0803
rank (out of 24 spectra)	9	6	8	5	4
quality test result	66.7	88.9	55.6	77.8	70.6

 
 
 

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# 2 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0001'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 01, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0001
type:		Measurement 02
NCBI ID (primary):		28450
NCBI ID (secondary):		28450
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia pseudomallei A101-10: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:27:14.015+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_01/0_H4/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	76.3746	146.1397	18	569.7561
rank (out of 24 spectra)	11	5	12	7	7
quality test result	66.7	100	44.4	77.8	69.4

 
 
 

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# 3 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0002'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0002
type:		Measurement 01
NCBI ID (primary):		119857
NCBI ID (secondary):		119857
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Heart cysteine agar (HCA)
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Francisella tularensis ssp. holarctica Ft 32: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:04:41.578+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_02/0_H5/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	41.6721	103.585	14	571.0471
rank (out of 24 spectra)	2	1	16	6	3
quality test result	77.8	100	44.4	77.8	72.2

 
 
 

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# 4 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0002'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 02, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0002
type:		Measurement 02
NCBI ID (primary):		119857
NCBI ID (secondary):		119857
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Heart cysteine agar (HCA)
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Francisella tularensis ssp. holarctica Ft 32: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:33:44.531+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_02/0_H6/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	83.1494	244.769	11	493.5037
rank (out of 24 spectra)	13	10	18	13	14
quality test result	55.6	77.8	33.3	66.7	56.1

 
 
 

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# 5 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0003'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0003
type:		Measurement 01
NCBI ID (primary):		36855
NCBI ID (secondary):		36855
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Brucella canis A138-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:11:32.906+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_03/0_H7/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	49.2393	106.8302	12	562.0953
rank (out of 24 spectra)	4	2	17	9	6
quality test result	77.8	100	33.3	66.7	66.1

 
 
 

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# 6 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0003'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 03, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0003
type:		Measurement 02
NCBI ID (primary):		36855
NCBI ID (secondary):		36855
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Brucella canis A138-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:42:40.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_03/0_H8/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	54.7129	252.9022	10	562.2198
rank (out of 24 spectra)	6	11	21	8	11
quality test result	66.7	77.8	33.3	66.7	58.9

 
 
 

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# 7 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0001'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0001
type:		Measurement 01
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:21:01.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_E19/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	153.799	1153.2979	27	430.1935
rank (out of 24 spectra)	18	19	1	17	15
quality test result	44.4	11.1	55.6	55.6	43.9

 
 
 

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# 8 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0004'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0004
type:		Measurement 02
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T08:52:26.953+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_H10/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	209.0902	1415.4679	10	326.4092
rank (out of 24 spectra)	21	23	20	22	23
quality test result	33.3	11.1	33.3	33.3	28.9

 
 
 

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# 9 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0004'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 04, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0004
type:		Measurement 03
NCBI ID (primary):		1392
NCBI ID (secondary):		1392
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus anthracis AMES: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:19:29.671+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_04/0_H9/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	178.0172	1199.374	24	455.1178
rank (out of 24 spectra)	20	20	2	16	17
quality test result	44.4	11.1	55.6	55.6	43.9

 
 
 

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# 10 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0005'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0005
type:		Measurement 01
NCBI ID (primary):		529
NCBI ID (secondary):		529
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Ochrobactrum anthropi A-148-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:25:30.359+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_05/0_H11/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	79.1949	265.1086	21	526.8862
rank (out of 24 spectra)	12	12	7	11	10
quality test result	66.7	77.8	55.6	66.7	65.6

 
 
 

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# 11 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0005'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 05, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0005
type:		Measurement 02
NCBI ID (primary):		529
NCBI ID (secondary):		529
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Ochrobactrum anthropi A-148-11: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:00:58.531+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_05/0_H12/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	93.8244	698.5319	23	495.4062
rank (out of 24 spectra)	15	18	3	12	12
quality test result	55.6	33.3	55.6	66.7	53.9

 
 
 

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# 12 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0006'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0006
type:		Measurement 01
NCBI ID (primary):		633
NCBI ID (secondary):		633
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pseudotuberculosis III: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:36:26.328+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_06/0_H13/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	75.1419	189.0819	21	635.197
rank (out of 24 spectra)	10	7	6	1	2
quality test result	66.7	88.9	55.6	77.8	70.6

 
 
 

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# 13 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0006'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 06, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0006
type:		Measurement 02
NCBI ID (primary):		633
NCBI ID (secondary):		633
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pseudotuberculosis III: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:22:37.468+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_06/0_H14/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	85.7281	429.542	21	603.455
rank (out of 24 spectra)	14	14	5	3	8
quality test result	55.6	55.6	55.6	77.8	61.1

 
 
 

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# 14 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0007'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0007
type:		Measurement 01
NCBI ID (primary):		13373
NCBI ID (secondary):		13373
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia mallei A106-3: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:39:43.625+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_07/0_H15/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	56.3846	110.6262	18	616.0892
rank (out of 24 spectra)	7	3	11	2	1
quality test result	66.7	100	44.4	77.8	69.4

 
 
 

---

   

# 15 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0007'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 07, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0007
type:		Measurement 02
NCBI ID (primary):		13373
NCBI ID (secondary):		13373
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia mallei A106-3: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:30:51.015+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_07/0_H16/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	69.0398	122.3824	14	590.3212
rank (out of 24 spectra)	8	4	15	4	5
quality test result	66.7	100	44.4	77.8	69.4

 
 
 

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# 16 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0002'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0002
type:		Measurement 01
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 30 mkL 100§ TFA, final TFA conc. approx. 80 perc.; 10 mkL 80 perc. TFA added; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:23:57.843+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_E20/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	176.345	487.5961	18	475.2536
rank (out of 24 spectra)	19	15	10	15	19
quality test result	44.4	44.4	44.4	55.6	47.2

 
 
 

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# 17 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0008'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0008
type:		Measurement 02
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:48:34.328+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_H17/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	94.8866	218.8987	21	538.2527
rank (out of 24 spectra)	16	8	4	10	9
quality test result	55.6	77.8	55.6	66.7	62.8

 
 
 

---

   

# 18 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0008'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 08, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0008
type:		Measurement 03
NCBI ID (primary):		57975
NCBI ID (secondary):		57975
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 20 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Burkholderia thailandensis E125: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:36:55.281+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_08/0_H18/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	134.4105	638.5213	20	488.4088
rank (out of 24 spectra)	17	16	9	14	16
quality test result	44.4	33.3	55.6	66.7	51.1

 
 
 

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# 19 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0003'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0003
type:		Measurement 01
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 30 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:29:00.578+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_E21/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	49.8658	689.8069	7	315.7638
rank (out of 24 spectra)	5	17	23	23	20
quality test result	77.8	33.3	22.2	33.3	41.1

 
 
 

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# 20 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0009'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0009
type:		Measurement 02
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 15 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T14:57:12.250+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_H19/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	34.2306	229.5792	7	403.8853
rank (out of 24 spectra)	1	9	22	20	13
quality test result	77.8	77.8	22.2	55.6	55.6

 
 
 

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# 21 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0009'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 09, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0009
type:		Measurement 03
NCBI ID (primary):		632
NCBI ID (secondary):		632
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 15 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Yersinia pestis A106-2: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:44:17.781+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_09/0_H20/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	48.6235	411.2283	6	417.52
rank (out of 24 spectra)	3	13	24	18	18
quality test result	77.8	55.6	11.1	55.6	47.8

 
 
 

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# 22 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_17_Maren_Stämmler_0004'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_17_Maren_Stämmler_0004
type:		Measurement 01
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 80 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-17T09:39:08.984+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_E22/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	290.2346	1370.9628	16	369.9011
rank (out of 24 spectra)	24	22	13	21	22
quality test result	33.3	11.1	44.4	44.4	35

 
 
 

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# 23 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_13_Maren_Stämmler_0010'

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Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_13_Maren_Stämmler_0010
type:		Measurement 02
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-13T15:05:30.796+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_H21/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	226.409	1244.7313	15	405.4401
rank (out of 24 spectra)	22	21	14	19	21
quality test result	33.3	11.1	44.4	55.6	37.8

 
 
 

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# 24 - QUALITY TEST REPORT FOR MALDI-TOF MS SPECTRUM '2013_06_14_Maren_Stämmler_0010'

'  

Metadata of actual MALDI-ToF test spectrum
genus / species / strain:		RKI MALDI sample 10, QUANDHIP EQAE ring trial, strains provided by RKI | ZBS 2
file id:		2013_06_14_Maren_Stämmler_0010
type:		Measurement 03
NCBI ID (primary):		1428
NCBI ID (secondary):		1428
growth time:		Optimal growth time between 24 - 72h
growth temperature:		37°C
growth conditions:		Optimal aerobic or microaerophilic conditions
growth medium:		Columbia blood agar (Oxoid), 2nd passage on TSA or Caso agar, harvested by the 2nd passage
sample treatment:		Sample mixed with 25 mkL 100§ TFA, final TFA conc. approx. 80 perc.; approx. 30 Min treatment time; diluted 1:10 (vol); mixed with 1:1 HCCA|TA2(A)
spores:		No
concentration:		Pellet produced by centrifugation (1 x 5 Min, 15,000 rpm) of a 500 mkL cell suspension, gamma ray irradiated (30 kGy)
extra info:		Bacillus thuringiensis DSM 350: microbial preparation by ZBS 2| preparation for MALDI-ToF MS: M. Stämmler
calibration standard:		linear calibration using Escherichia coli DSM 3871
measurement method:		D:\Methods\flexControlMethods\MaierMethods\ToM_200ns_20130611.par
customer:		RKI ZBS 2| ZBS 6
measurement date/time:		2013-06-14T09:51:48.343+02:00
path to MS file:		/home/lasch/MATLAB/microbe test spectra/ring trial RKI spectra/Sample_10/0_H22/1/1SLin

 
 
 
 

Detailed quality test report
Parameter	Noise	Baseline error	Number of peaks	Mean resolving power	General Quality
absolut qt results	271.2059	1423.7414	10	290.1593
rank (out of 24 spectra)	23	24	19	24	24
quality test result	33.3	11.1	33.3	33.3	28.9

 
 
 

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